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We developed a peptide nucleic acid (PNA) probe targeting <jats:italic>Prevotella bivia<\/jats:italic>, a common BV-associated bacteria, and optimized a multiplex approach for detection of <jats:italic>Gardnerella<\/jats:italic> spp., <jats:italic>P. bivia<\/jats:italic> and <jats:italic>Fannyhessea vaginae<\/jats:italic>. Our <jats:italic>P. bivia<\/jats:italic> PNA probe specifically detected the target species, and the optimized multiplex approach was able to detect the presence of the three species in multi-species BV biofilms.<\/jats:p>","DOI":"10.1038\/s41522-023-00411-6","type":"journal-article","created":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T05:53:30Z","timestamp":1687499610000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Development of a Prevotella bivia PNA probe and a multiplex approach to detect three relevant species in bacterial vaginosis-associated biofilms"],"prefix":"10.1038","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7323-4025","authenticated-orcid":false,"given":"L\u00facia G. V.","family":"Sousa","sequence":"first","affiliation":[]},{"given":"Carina","family":"Almeida","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4005-3858","authenticated-orcid":false,"given":"Christina A.","family":"Muzny","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3365-3537","authenticated-orcid":false,"given":"Nuno","family":"Cerca","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,6,23]]},"reference":[{"key":"411_CR1","doi-asserted-by":"publisher","first-page":"631972","DOI":"10.3389\/fcimb.2021.631972","volume":"11","author":"X Chen","year":"2021","unstructured":"Chen, X., Lu, Y., Chen, T. & Li, R. The Female Vaginal Microbiome in Health and Bacterial Vaginosis. Front. Cell. Infect. Microbiol. 11, 631972 (2021).","journal-title":"Front. Cell. Infect. 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Microbiology 156, 392\u2013399 (2010).","journal-title":"Microbiology"},{"key":"411_CR4","doi-asserted-by":"publisher","DOI":"10.1038\/srep11640","volume":"5","author":"J Castro","year":"2015","unstructured":"Castro, J. et al. Using an in-vitro biofilm model to assess the virulence potential of Bacterial Vaginosis or non-Bacterial Vaginosis Gardnerella vaginalis isolates. Sci. Rep. 5, 11640 (2015).","journal-title":"Sci. Rep."},{"key":"411_CR5","doi-asserted-by":"publisher","first-page":"1812","DOI":"10.1128\/JCM.00851-09","volume":"48","author":"M Zozaya-Hinchliffe","year":"2010","unstructured":"Zozaya-Hinchliffe, M., Lillis, R., Martin, D. H. & Ferris, M. J. Quantitative PCR assessments of bacterial species in women with and without bacterial vaginosis. J. Clin. Microbiol. 48, 1812\u20131819 (2010).","journal-title":"J. Clin. 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The other authors have no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"42"}}