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Yet, the role of integrins as vascular initiators in engineered materials is still not well understood. Here, we show that \u03b1v\u03b23 integrin-specific 3D matrices were able to retain PECAM1<jats:sup>+<\/jats:sup> cells from the stromal vascular fraction (SVF) of adipose tissue, triggering vasculogenesis in vitro in the absence of extrinsic growth factors. Our results suggest that \u03b1v\u03b23-RGD-driven signaling in the formation of capillary-like structures prevents the activation of the caspase 8 pathway and activates the FAK\/paxillin pathway, both responsible for endothelial cells (ECs) survival and migration. We also show that prevascularized \u03b1v\u03b23 integrin-specific constructs inosculate with the host vascular system fostering in vivo neovascularization. Overall, this work demonstrates the ability of the biomaterial to trigger vasculogenesis in an integrin-specific manner, by activating essential pathways for EC survival and migration within a self-regulatory growth factor microenvironment. This strategy represents an improvement to current vascularization routes for Tissue Engineering constructs, potentially enhancing their clinical applicability.<\/jats:p>","DOI":"10.1038\/s41536-022-00253-4","type":"journal-article","created":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T20:19:42Z","timestamp":1664309982000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Integrin-specific hydrogels for growth factor-free vasculogenesis"],"prefix":"10.1038","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5588-374X","authenticated-orcid":false,"given":"Helena R.","family":"Moreira","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0560-2962","authenticated-orcid":false,"given":"Daniel B.","family":"Rodrigues","sequence":"additional","affiliation":[]},{"given":"Sara","family":"Freitas-Ribeiro","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4889-7799","authenticated-orcid":false,"given":"Luc\u00edlia P.","family":"da Silva","sequence":"additional","affiliation":[]},{"given":"Alain","family":"da S. 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