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Most existing neural taxonomies are based on either transcriptomic\n                    <jats:sup>3,4<\/jats:sup>\n                    or morpho-electric\n                    <jats:sup>5,6<\/jats:sup>\n                    criteria, as it has been technically challenging to study both aspects of neuronal diversity in the same set of cells\n                    <jats:sup>7<\/jats:sup>\n                    . Here we used Patch-seq\n                    <jats:sup>8<\/jats:sup>\n                    to combine patch-clamp recording, biocytin staining, and single-cell RNA sequencing of more than 1,300 neurons in adult mouse primary motor cortex, providing a morpho-electric annotation of almost all transcriptomically defined neural cell types. We found that, although broad families of transcriptomic types (those expressing\n                    <jats:italic>Vip<\/jats:italic>\n                    ,\n                    <jats:italic>Pvalb<\/jats:italic>\n                    ,\n                    <jats:italic>Sst<\/jats:italic>\n                    and so on) had distinct and essentially non-overlapping morpho-electric phenotypes, individual transcriptomic types within the same family were not well separated in the morpho-electric space. Instead, there was a continuum of variability in morphology and electrophysiology, with neighbouring transcriptomic cell types showing similar morpho-electric features, often without clear boundaries between them. Our results suggest that neuronal types in the neocortex do not always form discrete entities. Instead, neurons form a hierarchy that consists of distinct non-overlapping branches at the level of families, but can form continuous and correlated transcriptomic and morpho-electrical landscapes within families.\n                  <\/jats:p>","DOI":"10.1038\/s41586-020-2907-3","type":"journal-article","created":{"date-parts":[[2020,11,12]],"date-time":"2020-11-12T12:05:45Z","timestamp":1605182745000},"page":"144-150","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":324,"title":["Phenotypic variation of transcriptomic cell types in mouse motor cortex"],"prefix":"10.1038","volume":"598","author":[{"given":"Federico","family":"Scala","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5639-7209","authenticated-orcid":false,"given":"Dmitry","family":"Kobak","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4458-117X","authenticated-orcid":false,"given":"Matteo","family":"Bernabucci","sequence":"additional","affiliation":[]},{"given":"Yves","family":"Bernaerts","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1963-8285","authenticated-orcid":false,"given":"Cathryn Ren\u00e9","family":"Cadwell","sequence":"additional","affiliation":[]},{"given":"Jesus Ramon","family":"Castro","sequence":"additional","affiliation":[]},{"given":"Leonard","family":"Hartmanis","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8066-1383","authenticated-orcid":false,"given":"Xiaolong","family":"Jiang","sequence":"additional","affiliation":[]},{"given":"Sophie","family":"Laturnus","sequence":"additional","affiliation":[]},{"given":"Elanine","family":"Miranda","sequence":"additional","affiliation":[]},{"given":"Shalaka","family":"Mulherkar","sequence":"additional","affiliation":[]},{"given":"Zheng Huan","family":"Tan","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9361-5607","authenticated-orcid":false,"given":"Zizhen","family":"Yao","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0326-5878","authenticated-orcid":false,"given":"Hongkui","family":"Zeng","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6473-1740","authenticated-orcid":false,"given":"Rickard","family":"Sandberg","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0199-4727","authenticated-orcid":false,"given":"Philipp","family":"Berens","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4305-6376","authenticated-orcid":false,"given":"Andreas S.","family":"Tolias","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2020,11,12]]},"reference":[{"key":"2907_CR1","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1038\/nn.3917","volume":"18","author":"KD Harris","year":"2015","unstructured":"Harris, K. 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