{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T13:19:03Z","timestamp":1770815943215,"version":"3.50.1"},"reference-count":49,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2020,10,26]],"date-time":"2020-10-26T00:00:00Z","timestamp":1603670400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,10,26]],"date-time":"2020-10-26T00:00:00Z","timestamp":1603670400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Chem Biol"],"published-print":{"date-parts":[[2021,2]]},"DOI":"10.1038\/s41589-020-00672-8","type":"journal-article","created":{"date-parts":[[2020,10,26]],"date-time":"2020-10-26T17:03:18Z","timestamp":1603731798000},"page":"196-204","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Interhelical interactions within the STIM1 CC1 domain modulate CRAC channel activation"],"prefix":"10.1038","volume":"17","author":[{"given":"Petr","family":"Rathner","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2689-0158","authenticated-orcid":false,"given":"Marc","family":"Fahrner","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0795-9594","authenticated-orcid":false,"given":"Linda","family":"Cerofolini","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0870-5833","authenticated-orcid":false,"given":"Herwig","family":"Grabmayr","sequence":"additional","affiliation":[]},{"given":"Ferdinand","family":"Horvath","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6473-8109","authenticated-orcid":false,"given":"Heinrich","family":"Krobath","sequence":"additional","affiliation":[]},{"given":"Agrim","family":"Gupta","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7708-9208","authenticated-orcid":false,"given":"Enrico","family":"Ravera","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8440-1690","authenticated-orcid":false,"given":"Marco","family":"Fragai","sequence":"additional","affiliation":[]},{"given":"Matthias","family":"Bechmann","sequence":"additional","affiliation":[]},{"given":"Thomas","family":"Renger","sequence":"additional","affiliation":[]},{"given":"Claudio","family":"Luchinat","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3756-4136","authenticated-orcid":false,"given":"Christoph","family":"Romanin","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7621-3980","authenticated-orcid":false,"given":"Norbert","family":"M\u00fcller","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2020,10,26]]},"reference":[{"key":"672_CR1","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1242\/jcs.114.12.2213","volume":"114","author":"MD Bootman","year":"2001","unstructured":"Bootman, M. D., Lipp, P. & Berridge, M. J. The organisation and functions of local Ca(2+) signals. J. Cell Sci. 114, 2213\u20132222 (2001).","journal-title":"J. Cell Sci."},{"key":"672_CR2","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1038\/nrm1155","volume":"4","author":"MJ Berridge","year":"2003","unstructured":"Berridge, M. J., Bootman, M. D. & Roderick, H. L. Calcium signalling: dynamics, homeostasis and remodelling. Nat. Rev. Mol. Cell Biol. 4, 517\u2013529 (2003).","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"672_CR3","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.bbamcr.2008.10.005","volume":"1793","author":"MJ Berridge","year":"2009","unstructured":"Berridge, M. J. Inositol trisphosphate and calcium signalling mechanisms. Biochim. Biophys. Acta 1793, 933\u2013940 (2009).","journal-title":"Biochim. Biophys. Acta"},{"key":"672_CR4","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/nyas.12938","volume":"1356","author":"RS Lacruz","year":"2015","unstructured":"Lacruz, R. S. & Feske, S. Diseases caused by mutations in ORAI1 and STIM1. Ann. N.Y. Acad. Sci. 1356, 45\u201379 (2015).","journal-title":"Ann. N.Y. Acad. Sci."},{"key":"672_CR5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0143-4160(86)90026-6","volume":"7","author":"JW Putney Jr.","year":"1986","unstructured":"Putney, J. W. Jr. A model for receptor-regulated calcium entry. Cell Calcium 7, 1\u201312 (1986).","journal-title":"Cell Calcium"},{"key":"672_CR6","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1038\/nature04147","volume":"437","author":"SL Zhang","year":"2005","unstructured":"Zhang, S. L. et al. STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane. Nature 437, 902\u2013905 (2005).","journal-title":"Nature"},{"key":"672_CR7","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1038\/nature04702","volume":"441","author":"S Feske","year":"2006","unstructured":"Feske, S. et al. A mutation in orai1 causes immune deficiency by abrogating CRAC channel function. Nature 441, 179\u2013185 (2006).","journal-title":"Nature"},{"key":"672_CR8","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1038\/nature05108","volume":"443","author":"AV Yeromin","year":"2006","unstructured":"Yeromin, A. V. et al. Molecular identification of the CRAC channel by altered ion selectivity in a mutant of orai. Nature 443, 226\u2013229 (2006).","journal-title":"Nature"},{"key":"672_CR9","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1016\/j.cub.2005.05.055","volume":"15","author":"J Liou","year":"2005","unstructured":"Liou, J. et al. STIM is a Ca2+ sensor essential for Ca2+-store-depletion-triggered Ca2+ influx. Curr. Biol. 15, 1235\u20131241 (2005).","journal-title":"Curr. Biol."},{"key":"672_CR10","doi-asserted-by":"crossref","first-page":"16704","DOI":"10.1073\/pnas.0608358103","volume":"103","author":"Y Baba","year":"2006","unstructured":"Baba, Y. et al. Coupling of STIM1 to store-operated Ca2+ entry through its constitutive and inducible movement in the endoplasmic reticulum. Proc. Natl Acad. Sci. USA 103, 16704\u201316709 (2006).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"672_CR11","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1038\/nature07065","volume":"454","author":"RM Luik","year":"2008","unstructured":"Luik, R. M., Wang, B., Prakriya, M., Wu, M. M. & Lewis, R. S. Oligomerization of STIM1 couples ER calcium depletion to CRAC channel activation. Nature 454, 538\u2013542 (2008).","journal-title":"Nature"},{"key":"672_CR12","doi-asserted-by":"crossref","first-page":"5383","DOI":"10.1113\/jphysiol.2008.162503","volume":"586","author":"L Navarro-Borelly","year":"2008","unstructured":"Navarro-Borelly, L. et al. STIM1-Orai1 interactions and orai1 conformational changes revealed by live-cell FRET microscopy. J. Physiol. 586, 5383\u20135401 (2008).","journal-title":"J. Physiol."},{"key":"672_CR13","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1139\/O09-125","volume":"88","author":"PB Stathopulos","year":"2010","unstructured":"Stathopulos, P. B. & Ikura, M. Partial unfolding and oligomerization of stromal interaction molecules as an initiation mechanism of store operated calcium entry. Biochem. Cell Biol. 88, 175\u2013183 (2010).","journal-title":"Biochem. Cell Biol."},{"key":"672_CR14","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.1038\/emboj.2011.79","volume":"30","author":"M Muik","year":"2011","unstructured":"Muik, M. et al. STIM1 couples to ORAI1 via an intramolecular transition into an extended conformation. EMBO J. 30, 1678\u20131689 (2011).","journal-title":"EMBO J."},{"key":"672_CR15","doi-asserted-by":"crossref","first-page":"33231","DOI":"10.1074\/jbc.M114.610022","volume":"289","author":"M Fahrner","year":"2014","unstructured":"Fahrner, M. et al. A coiled-coil clamp controls both conformation and clustering of stromal interaction molecule 1 (STIM1). J. Biol. Chem. 289, 33231\u201333244 (2014).","journal-title":"J. Biol. Chem."},{"key":"672_CR16","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1038\/ncb1842","volume":"11","author":"JP Yuan","year":"2009","unstructured":"Yuan, J. P. et al. SOAR and the polybasic STIM1 domains gate and regulate ORAI channels. Nat. Cell Biol. 11, 337\u2013343 (2009).","journal-title":"Nat. Cell Biol."},{"key":"672_CR17","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1016\/j.cell.2009.02.014","volume":"136","author":"CY Park","year":"2009","unstructured":"Park, C. Y. et al. STIM1 clusters and activates CRAC channels via direct binding of a cytosolic domain to orai1. Cell 136, 876\u2013890 (2009).","journal-title":"Cell"},{"key":"672_CR18","doi-asserted-by":"crossref","first-page":"8421","DOI":"10.1074\/jbc.C800229200","volume":"284","author":"M Muik","year":"2009","unstructured":"Muik, M. et al. A cytosolic homomerization and a modulatory domain within STIM1 C terminus determine coupling to ORAI1 channels. J. Biol. Chem. 284, 8421\u20138426 (2009).","journal-title":"J. Biol. Chem."},{"key":"672_CR19","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1002\/humu.22621","volume":"35","author":"G Morin","year":"2014","unstructured":"Morin, G. et al. Gain-of-Function Mutation in STIM1 (P.R304W) is associated with Stormorken syndrome. Hum. Mutat. 35, 1221\u20131232 (2014).","journal-title":"Hum. Mutat."},{"key":"672_CR20","doi-asserted-by":"crossref","first-page":"4197","DOI":"10.1073\/pnas.1312520111","volume":"111","author":"V Nesin","year":"2014","unstructured":"Nesin, V. et al. Activating mutations in STIM1 and ORAI1 cause overlapping syndromes of tubular myopathy and congenital miosis. Proc. Natl Acad. Sci. USA 111, 4197\u20134202 (2014).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"672_CR21","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1038\/s41467-018-03062-w","volume":"9","author":"M Fahrner","year":"2018","unstructured":"Fahrner, M. et al. A dual mechanism promotes switching of the Stormorken STIM1 R304W mutant into the activated state. Nat. Commun. 9, 825\u2013825 (2018).","journal-title":"Nat. Commun."},{"key":"672_CR22","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1002\/humu.22544","volume":"35","author":"D Misceo","year":"2014","unstructured":"Misceo, D. et al. A dominant STIM1 mutation causes Stormorken syndrome. Hum. Mutat. 35, 556\u2013564 (2014).","journal-title":"Hum. Mutat."},{"key":"672_CR23","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.pep.2018.01.013","volume":"146","author":"P Rathner","year":"2018","unstructured":"Rathner, P. et al. Rapid NMR-scale purification of (15)N,(13)C isotope-labeled recombinant human STIM1 coiled coil fragments. Protein Expr. Purif. 146, 45\u201350 (2018).","journal-title":"Protein Expr. Purif."},{"key":"672_CR24","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1126\/science.252.5009.1162","volume":"252","author":"A Lupas","year":"1991","unstructured":"Lupas, A., Van Dyke, M. & Stock, J. Predicting coiled coils from protein sequences. Science 252, 1162\u20131164 (1991).","journal-title":"Science"},{"key":"672_CR25","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1038\/170882b0","volume":"170","author":"FHC Crick","year":"1952","unstructured":"Crick, F. H. C. Is \u03b1-Keratin a coiled coil? Nature 170, 882\u2013883 (1952).","journal-title":"Nature"},{"key":"672_CR26","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1107\/S0365110X53001964","volume":"6","author":"FHC Crick","year":"1953","unstructured":"Crick, F. H. C. The packing of \u03b1-helices: simple coiled-coils. Acta Crystallogr. 6, 689\u2013697 (1953).","journal-title":"Acta Crystallogr."},{"key":"672_CR27","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0065-3233(05)70003-6","volume":"70","author":"AN Lupas","year":"2005","unstructured":"Lupas, A. N. & Gruber, M. The structure of alpha-helical coiled coils. Adv. Protein Chem. 70, 37\u201378 (2005).","journal-title":"Adv. Protein Chem."},{"key":"672_CR28","doi-asserted-by":"crossref","first-page":"D402","DOI":"10.1093\/nar\/gkm957","volume":"36","author":"EL Ulrich","year":"2007","unstructured":"Ulrich, E. L. et al. BioMagResBank. Nucleic Acids Res. 36, D402\u2013D408 (2007).","journal-title":"Nucleic Acids Res."},{"key":"672_CR29","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1006\/jmbi.1997.1284","volume":"273","author":"P G\u00fcntert","year":"1997","unstructured":"G\u00fcntert, P., Mumenthaler, C. & W\u00fcthrich, K. Torsion angle dynamics for NMR structure calculation with the new program DYANA. J. Mol. Biol. 273, 283\u2013298 (1997).","journal-title":"J. Mol. Biol."},{"key":"672_CR30","first-page":"353","volume":"278","author":"P G\u00fcntert","year":"2004","unstructured":"G\u00fcntert, P. Automated NMR structure calculation with CYANA. Methods Mol. Biol. 278, 353\u2013378 (2004).","journal-title":"Methods Mol. Biol."},{"key":"672_CR31","doi-asserted-by":"crossref","first-page":"10873","DOI":"10.1073\/pnas.1203013109","volume":"109","author":"OF Lange","year":"2012","unstructured":"Lange, O. F. et al. Determination of solution structures of proteins up to 40 kDa using CS-Rosetta with sparse NMR data from deuterated samples. Proc. Natl Acad. Sci. USA 109, 10873\u201310878 (2012).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"672_CR32","doi-asserted-by":"crossref","first-page":"e74735","DOI":"10.1371\/journal.pone.0074735","volume":"8","author":"B Cui","year":"2013","unstructured":"Cui, B. et al. The inhibitory helix controls the intramolecular conformational switching of the C-terminus of STIM1. PLoS ONE 8, e74735 (2013).","journal-title":"PLoS ONE"},{"key":"672_CR33","unstructured":"Limbach, H.-H., Ratajczak, H. & Orville-Thomas, W. J. Molecular Interactions Vol. 1 (John Wiley & Sons Ltd, 1980)."},{"key":"672_CR34","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1146\/annurev.biophys.30.1.129","volume":"30","author":"AG Palmer 3rd","year":"2001","unstructured":"Palmer, A. G. 3rd Nmr probes of molecular dynamics: overview and comparison with other techniques. Annu. Rev. Biophys. Biomol. Struct. 30, 129\u2013155 (2001).","journal-title":"Annu. Rev. Biophys. Biomol. Struct."},{"key":"672_CR35","volume":"6","author":"G Ma","year":"2015","unstructured":"Ma, G. et al. Inside-out Ca signalling prompted by STIM1 conformational switch. Nat. Commun. 6, 7826 (2015).","journal-title":"Nat. Commun."},{"key":"672_CR36","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/s10858-013-9741-y","volume":"56","author":"Y Shen","year":"2013","unstructured":"Shen, Y. & Bax, A. Protein backbone and sidechain torsion angles predicted from NMR chemical shifts using artificial neural networks. J. Biomol. NMR 56, 227\u2013241 (2013).","journal-title":"J. Biomol. NMR"},{"key":"672_CR37","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms3963","volume":"4","author":"PB Stathopulos","year":"2013","unstructured":"Stathopulos, P. B. et al. STIM1\/Orai1 coiled-coil interplay in the regulation of store-operated calcium entry. Nat. Commun. 4, 2963 (2013).","journal-title":"Nat. Commun."},{"key":"672_CR38","doi-asserted-by":"crossref","first-page":"8972","DOI":"10.1021\/bi00449a003","volume":"28","author":"LE Kay","year":"1989","unstructured":"Kay, L. E., Torchia, D. A. & Bax, A. Backbone dynamics of proteins as studied by 15N inverse detected heteronuclear NMR spectroscopy: application to staphylococcal nuclease. Biochemistry 28, 8972\u20138979 (1989).","journal-title":"Biochemistry"},{"key":"672_CR39","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1107\/S0021889892009944","volume":"26","author":"RA Laskowski","year":"1993","unstructured":"Laskowski, R. A., MacArthur, M. W., Moss, D. S. & Thornton, J. M. PROCHECK: a program to check the stereochemical quality of protein structures. J. Appl. Crystallogr. 26, 283\u2013291 (1993).","journal-title":"J. Appl. Crystallogr."},{"key":"672_CR40","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.celrep.2017.12.030","volume":"22","author":"N Hirve","year":"2018","unstructured":"Hirve, N., Rajanikanth, V., Hogan, P. G. & Gudlur, A. Coiled-coil formation conveys a STIM1 signal from ER lumen to cytoplasm. Cell Rep. 22, 72\u201383 (2018).","journal-title":"Cell Rep."},{"key":"672_CR41","volume":"5","author":"J Luo","year":"2015","unstructured":"Luo, J., Liu, Z., Guo, Y. & Li, M. A structural dissection of large protein\u2013protein crystal packing contacts. Sci. Rep. 5, 14214 (2015).","journal-title":"Sci. Rep."},{"key":"672_CR42","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1038\/s41467-020-14841-9","volume":"11","author":"G Ma","year":"2020","unstructured":"Ma, G. et al. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat. Commun. 11, 1039\u20131039 (2020).","journal-title":"Nat. Commun."},{"key":"672_CR43","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/s10858-010-9459-z","volume":"49","author":"B Krishnarjuna","year":"2011","unstructured":"Krishnarjuna, B., Jaipuria, G., Thakur, A., D\u2019Silva, P. & Atreya, H. S. Amino acid selective unlabeling for sequence specific resonance assignments in proteins. J. Biomol. NMR 49, 39\u201351 (2011).","journal-title":"J. Biomol. NMR"},{"key":"672_CR44","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1080\/152165401317291147","volume":"52","author":"V Kanelis","year":"2001","unstructured":"Kanelis, V., Forman-Kay, J. D. & Kay, L. E. Multidimensional NMR methods for protein structure determination. IUBMB Life 52, 291\u2013302 (2001).","journal-title":"IUBMB Life"},{"key":"672_CR45","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1002\/cmr.a.20109","volume":"32A","author":"W Bermel","year":"2008","unstructured":"Bermel, W., Felli, I., K\u00fcmmerle, R. & Pierattelli, R. 13C Direct\u2010detection biomolecular NMR. Concepts Magn. Reson. A. 32A, 183\u2013200 (2008).","journal-title":"Concepts Magn. Reson. A."},{"key":"672_CR46","doi-asserted-by":"crossref","first-page":"2981","DOI":"10.1093\/bioinformatics\/btu426","volume":"30","author":"E Krieger","year":"2014","unstructured":"Krieger, E. & Vriend, G. YASARA View - molecular graphics for all devices - from smartphones to workstations. Bioinforma 30, 2981\u20132982 (2014).","journal-title":"Bioinforma"},{"key":"672_CR47","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1002\/prot.10104","volume":"47","author":"E Krieger","year":"2002","unstructured":"Krieger, E., Koraimann, G. & Vriend, G. Increasing the precision of comparative models with YASARA NOVA\u2013a self-parameterizing force field. Proteins 47, 393\u2013402 (2002).","journal-title":"Proteins"},{"key":"672_CR48","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1113\/jphysiol.2006.118661","volume":"577","author":"I Derler","year":"2006","unstructured":"Derler, I. et al. Dynamic but not constitutive association of calmodulin with rat TRPV6 channels enables fine tuning of Ca2+-dependent inactivation. J. Physiol. 577, 31\u201344 (2006).","journal-title":"J. Physiol."},{"key":"672_CR49","doi-asserted-by":"crossref","first-page":"3923","DOI":"10.1529\/biophysj.103.022087","volume":"86","author":"T Zal","year":"2004","unstructured":"Zal, T. & Gascoigne, N. R. Photobleaching-corrected FRET efficiency imaging of live cells. Biophys. J. 86, 3923\u20133939 (2004).","journal-title":"Biophys. J."}],"container-title":["Nature Chemical Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41589-020-00672-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41589-020-00672-8","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41589-020-00672-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T23:55:28Z","timestamp":1635206128000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41589-020-00672-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,26]]},"references-count":49,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["672"],"URL":"https:\/\/doi.org\/10.1038\/s41589-020-00672-8","relation":{},"ISSN":["1552-4450","1552-4469"],"issn-type":[{"value":"1552-4450","type":"print"},{"value":"1552-4469","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,26]]},"assertion":[{"value":"16 April 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 September 2020","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 September 2020","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 October 2020","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}