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Because a large part of our knowledge rests on computational predictions, it is crucial that their accuracy is high. The Critical Assessment of protein Intrinsic Disorder prediction (CAID) experiment was established as a community-based blind test to determine the state of the art in prediction of intrinsically disordered regions and the subset of residues involved in binding. A total of 43\u2009methods were evaluated on a dataset of 646\u2009proteins from DisProt. The best methods use deep learning techniques and notably outperform physicochemical methods. The top disorder predictor has\n                    <jats:italic>F<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    \u2009=\u20090.483 on the full dataset and\n                    <jats:italic>F<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    \u2009=\u20090.792 following filtering out of bona fide structured regions. Disordered binding regions remain hard to predict, with\n                    <jats:italic>F<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    \u2009=\u20090.231. Interestingly, computing times among methods can vary by up to four orders of magnitude.\n                  <\/jats:p>","DOI":"10.1038\/s41592-021-01117-3","type":"journal-article","created":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T12:03:24Z","timestamp":1618833804000},"page":"472-481","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":331,"title":["Critical assessment of protein intrinsic disorder prediction"],"prefix":"10.1038","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9377-482X","authenticated-orcid":false,"given":"Marco","family":"Necci","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8210-2390","authenticated-orcid":false,"given":"Damiano","family":"Piovesan","sequence":"additional","affiliation":[]},{"name":"CAID Predictors","sequence":"additional","affiliation":[]},{"given":"Md Tamjidul","family":"Hoque","sequence":"additional","affiliation":[]},{"given":"Ian","family":"Walsh","sequence":"additional","affiliation":[]},{"given":"Sumaiya","family":"Iqbal","sequence":"additional","affiliation":[]},{"given":"Michele","family":"Vendruscolo","sequence":"additional","affiliation":[]},{"given":"Pietro","family":"Sormanni","sequence":"additional","affiliation":[]},{"given":"Chen","family":"Wang","sequence":"additional","affiliation":[]},{"given":"Daniele","family":"Raimondi","sequence":"additional","affiliation":[]},{"given":"Ronesh","family":"Sharma","sequence":"additional","affiliation":[]},{"given":"Yaoqi","family":"Zhou","sequence":"additional","affiliation":[]},{"given":"Thomas","family":"Litfin","sequence":"additional","affiliation":[]},{"given":"Oxana Valerianovna","family":"Galzitskaya","sequence":"additional","affiliation":[]},{"given":"Michail Yu.","family":"Lobanov","sequence":"additional","affiliation":[]},{"given":"Wim","family":"Vranken","sequence":"additional","affiliation":[]},{"given":"Bj\u00f6rn","family":"Wallner","sequence":"additional","affiliation":[]},{"given":"Claudio","family":"Mirabello","sequence":"additional","affiliation":[]},{"given":"Nawar","family":"Malhis","sequence":"additional","affiliation":[]},{"given":"Zsuzsanna","family":"Doszt\u00e1nyi","sequence":"additional","affiliation":[]},{"given":"G\u00e1bor","family":"Erd\u0151s","sequence":"additional","affiliation":[]},{"given":"B\u00e1lint","family":"M\u00e9sz\u00e1ros","sequence":"additional","affiliation":[]},{"given":"Jianzhao","family":"Gao","sequence":"additional","affiliation":[]},{"given":"Kui","family":"Wang","sequence":"additional","affiliation":[]},{"given":"Gang","family":"Hu","sequence":"additional","affiliation":[]},{"given":"Zhonghua","family":"Wu","sequence":"additional","affiliation":[]},{"given":"Alok","family":"Sharma","sequence":"additional","affiliation":[]},{"given":"Jack","family":"Hanson","sequence":"additional","affiliation":[]},{"given":"Kuldip","family":"Paliwal","sequence":"additional","affiliation":[]},{"given":"Isabelle","family":"Callebaut","sequence":"additional","affiliation":[]},{"given":"Tristan","family":"Bitard-Feildel","sequence":"additional","affiliation":[]},{"given":"Gabriele","family":"Orlando","sequence":"additional","affiliation":[]},{"given":"Zhenling","family":"Peng","sequence":"additional","affiliation":[]},{"given":"Jinbo","family":"Xu","sequence":"additional","affiliation":[]},{"given":"Sheng","family":"Wang","sequence":"additional","affiliation":[]},{"given":"David T.","family":"Jones","sequence":"additional","affiliation":[]},{"given":"Domenico","family":"Cozzetto","sequence":"additional","affiliation":[]},{"given":"Fanchi","family":"Meng","sequence":"additional","affiliation":[]},{"given":"Jing","family":"Yan","sequence":"additional","affiliation":[]},{"given":"J\u00f6rg","family":"Gsponer","sequence":"additional","affiliation":[]},{"given":"Jianlin","family":"Cheng","sequence":"additional","affiliation":[]},{"given":"Tianqi","family":"Wu","sequence":"additional","affiliation":[]},{"given":"Lukasz","family":"Kurgan","sequence":"additional","affiliation":[]},{"name":"DisProt Curators","sequence":"additional","affiliation":[]},{"given":"Vasilis J.","family":"Promponas","sequence":"additional","affiliation":[]},{"given":"Stella","family":"Tamana","sequence":"additional","affiliation":[]},{"given":"Cristina","family":"Marino-Buslje","sequence":"additional","affiliation":[]},{"given":"Elizabeth","family":"Mart\u00ednez-P\u00e9rez","sequence":"additional","affiliation":[]},{"given":"Anastasia","family":"Chasapi","sequence":"additional","affiliation":[]},{"given":"Christos","family":"Ouzounis","sequence":"additional","affiliation":[]},{"given":"A. 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