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The \u03b3\u03b4 T cell lineage is characterized by a signature \u03b3\u03b4 T cell receptor (\u03b3\u03b4TCR) that displays extensive sequence variability originated by DNA rearrangement of the corresponding V(D)J loci. Human \u03b3\u03b4 T cells comprise V\u03b39V\u03b42 T cells, the major subset in the peripheral blood; and V\u03b41<jats:sup>+<\/jats:sup> T cells, the predominant subpopulation in the post-natal thymus and in peripheral tissues. While less studied, V\u03b41<jats:sup>+<\/jats:sup> T cells recently gathered significant attention due to their anti-cancer and anti-viral activities. In this study we applied next-generation sequencing (NGS) to analyse the \u03b3\u03b4TCR repertoire of highly (FACS-)purified V\u03b41<jats:sup>+<\/jats:sup> T cells from human thymic biopsies. Our analysis reveals unsuspected aspects of thymically rearranged and expressed (at the mRNA level) <jats:italic>TRG<\/jats:italic> and <jats:italic>TRD<\/jats:italic> genes, thus constituting a data resource that qualifies previous conclusions on the TCR repertoire of \u03b3\u03b4 T cells developing in the human thymus.<\/jats:p>","DOI":"10.1038\/s41597-019-0118-2","type":"journal-article","created":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T14:24:03Z","timestamp":1562250243000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["High-throughput analysis of the human thymic V\u03b41+ T cell receptor repertoire"],"prefix":"10.1038","volume":"6","author":[{"given":"Biagio","family":"Di Lorenzo","sequence":"first","affiliation":[]},{"given":"Sarina","family":"Ravens","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4141-9302","authenticated-orcid":false,"given":"Bruno","family":"Silva-Santos","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2019,7,4]]},"reference":[{"key":"118_CR1","doi-asserted-by":"publisher","first-page":"929","DOI":"10.3389\/fimmu.2018.00929","volume":"9","author":"A Sim\u00f5es","year":"2018","unstructured":"Sim\u00f5es, A., Di Lorenzo, B. & Silva-Santos, B. 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The other authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"115"}}