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They are highly threatened showing the highest record of extinction events within faunal taxa. Conservation is particularly concerning in species occurring in the Mediterranean biodiversity hotspot that are exposed to multiple anthropogenic threats, possibly acting in synergy. That is the case of the dolphin freshwater mussel<jats:italic>Unio delphinus<\/jats:italic>Spengler, 1793, endemic to the western Iberian Peninsula with recently strong population declines. To date, only four genome assemblies are available for the order Unionida and only one European species. We present the first genome assembly of<jats:italic>Unio delphinus<\/jats:italic>. We used the PacBio HiFi to generate a highly contiguous genome assembly. The assembly is 2.5\u2009Gb long, possessing 1254 contigs with a contig N50 length of 10 Mbp. This is the most contiguous freshwater mussel genome assembly to date and is an essential resource for investigating the species\u2019 biology and evolutionary history that ultimately will help to support conservation strategies.<\/jats:p>","DOI":"10.1038\/s41597-023-02251-7","type":"journal-article","created":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T07:02:16Z","timestamp":1685602936000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["PacBio Hi-Fi genome assembly of the Iberian dolphin freshwater mussel Unio delphinus Spengler, 1793"],"prefix":"10.1038","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9973-4861","authenticated-orcid":false,"given":"Andr\u00e9","family":"Gomes-dos-Santos","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2761-7962","authenticated-orcid":false,"given":"Manuel","family":"Lopes-Lima","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6857-7581","authenticated-orcid":false,"given":"M. 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