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Cellulosome assembly involves the binding of enzyme-borne dockerins (Doc) to repeated cohesin (Coh) modules located in a non-catalytic scaffoldin. Docs appended to cellulosomal enzymes generally present two similar Coh-binding interfaces supporting a dual-binding mode, which may confer increased positional adjustment of the different complex components. <jats:italic>Ruminococcus flavefaciens<\/jats:italic>\u2019 cellulosome is assembled from a repertoire of 223 Doc-containing proteins classified into 6 groups. Recent studies revealed that Docs of groups 3 and 6 are recruited to the cellulosome <jats:italic>via<\/jats:italic> a single-binding mode mechanism with an adaptor scaffoldin. To investigate the extent to which the single-binding mode contributes to the assembly of <jats:italic>R<\/jats:italic>. <jats:italic>flavefaciens<\/jats:italic> cellulosome, the structures of two group 1 Docs bound to Cohs of primary (ScaA) and adaptor (ScaB) scaffoldins were solved. The data revealed that group 1 Docs display a conserved mechanism of Coh recognition involving a single-binding mode. Therefore, in contrast to all cellulosomes described to date, the assembly of <jats:italic>R<\/jats:italic>. <jats:italic>flavefaciens<\/jats:italic> cellulosome involves single but not dual-binding mode Docs. Thus, this work reveals a novel mechanism of cellulosome assembly and challenges the ubiquitous implication of the dual-binding mode in the acquisition of cellulosome flexibility.<\/jats:p>","DOI":"10.1038\/s41598-017-00919-w","type":"journal-article","created":{"date-parts":[[2017,4,3]],"date-time":"2017-04-03T21:27:07Z","timestamp":1491254827000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes"],"prefix":"10.1038","volume":"7","author":[{"given":"Pedro","family":"Bule","sequence":"first","affiliation":[]},{"given":"Victor D.","family":"Alves","sequence":"additional","affiliation":[]},{"given":"Vered","family":"Israeli-Ruimy","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3824-0240","authenticated-orcid":false,"given":"Ana L.","family":"Carvalho","sequence":"additional","affiliation":[]},{"given":"Lu\u00eds M. 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