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The deletion of NIPP1 in mice is early embryonic lethal, which has precluded functional studies in adult tissues. Hence, we have generated an inducible NIPP1 knockout model using a tamoxifen-inducible Cre recombinase transgene. The inactivation of the NIPP1 encoding alleles (<jats:italic>Ppp1r8<\/jats:italic>) in adult mice occurred very efficiently in testis and resulted in a gradual loss of germ cells, culminating in a Sertoli-cell only phenotype. Before the overt development of this phenotype <jats:italic>Ppp1r8<\/jats:italic><jats:sup>\n                  <jats:italic>\u2212\/\u2212<\/jats:italic>\n                <\/jats:sup> testis showed a decreased proliferation and survival capacity of cells of the spermatogenic lineage. A reduced proliferation was also detected after the tamoxifen-induced removal of NIPP1 from cultured testis slices and isolated germ cells enriched for undifferentiated spermatogonia, hinting at a testis-intrinsic defect. Consistent with the observed phenotype, RNA sequencing identified changes in the transcript levels of cell-cycle and apoptosis regulating genes in NIPP1-depleted testis. We conclude that NIPP1 is essential for mammalian spermatogenesis because it is indispensable for the proliferation and survival of progenitor germ cells, including (un)differentiated spermatogonia.<\/jats:p>","DOI":"10.1038\/s41598-017-13809-y","type":"journal-article","created":{"date-parts":[[2017,10,11]],"date-time":"2017-10-11T11:50:51Z","timestamp":1507722651000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["The protein phosphatase 1 regulator NIPP1 is essential for mammalian spermatogenesis"],"prefix":"10.1038","volume":"7","author":[{"given":"M\u00f3nica","family":"Ferreira","sequence":"first","affiliation":[]},{"given":"Shannah","family":"Boens","sequence":"additional","affiliation":[]},{"given":"Claudia","family":"Winkler","sequence":"additional","affiliation":[]},{"given":"Kathelijne","family":"Szek\u00e9r","sequence":"additional","affiliation":[]},{"given":"Iris","family":"Verbinnen","sequence":"additional","affiliation":[]},{"given":"Aleyde","family":"Van Eynde","sequence":"additional","affiliation":[]},{"given":"Margarida","family":"Fardilha","sequence":"additional","affiliation":[]},{"given":"Mathieu","family":"Bollen","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2017,10,17]]},"reference":[{"key":"13809_CR1","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1196\/annals.1411.003","volume":"1120","author":"S Yoshida","year":"2007","unstructured":"Yoshida, S., Nabeshima, Y. 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