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Here, we show that miR-214 expression is detected in bone and cartilage of zebrafish skeleton, and is downregulated during murine ATDC5 chondrocyte differentiation. Additionally, we observed a conservation of the transcriptional regulation of miR-214 primary transcript <jats:italic>Dnm3os<\/jats:italic> in vertebrates, being regulated by Ets1 in ATDC5 chondrogenic cells. Moreover, overexpression of miR-214 <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> mitigated chondrocyte differentiation probably by targeting activating transcription factor 4 (Atf4). Indeed, miR-214 overexpression <jats:italic>in vivo<\/jats:italic> hampered cranial cartilage formation of zebrafish and coincided with downregulation of <jats:italic>atf4<\/jats:italic> and of the key chondrogenic players <jats:italic>sox9<\/jats:italic> and <jats:italic>col2a1<\/jats:italic>. We show that miR-214 overexpression exerts a negative role in chondrogenesis by impacting on chondrocyte differentiation possibly through conserved mechanisms.<\/jats:p>","DOI":"10.1038\/s41598-018-21735-w","type":"journal-article","created":{"date-parts":[[2018,2,21]],"date-time":"2018-02-21T11:50:33Z","timestamp":1519213833000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Evidences for a New Role of miR-214 in Chondrogenesis"],"prefix":"10.1038","volume":"8","author":[{"given":"V\u00e2nia Palma","family":"Roberto","sequence":"first","affiliation":[]},{"given":"Paulo","family":"Gavaia","sequence":"additional","affiliation":[]},{"given":"Maria Jo\u00e3o","family":"Nunes","sequence":"additional","affiliation":[]},{"given":"Elsa","family":"Rodrigues","sequence":"additional","affiliation":[]},{"given":"Maria Leonor","family":"Cancela","sequence":"additional","affiliation":[]},{"given":"Daniel Martins","family":"Tiago","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2018,2,27]]},"reference":[{"key":"21735_CR1","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1177\/1759720X12448454","volume":"4","author":"MB Goldring","year":"2012","unstructured":"Goldring, M. 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