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In this context, <jats:italic>Tetraselmis<\/jats:italic> sp. CTP4 was successfully scaled up from an agar plate to 35- and 100-m<jats:sup>3<\/jats:sup> industrial scale tubular photobioreactors (PBR). Growth was performed semi-continuously for 60 days in the autumn-winter season (17<jats:sup>th<\/jats:sup> October \u2013 14<jats:sup>th<\/jats:sup> December). Optimisation of tubular PBR operations showed that improved productivities were obtained at a culture velocity of 0.65\u20131.35\u2009m\u2009s<jats:sup>\u22121<\/jats:sup> and a pH set-point for CO<jats:sub>2<\/jats:sub> injection of 8.0. Highest volumetric (0.08\u2009\u00b1\u20090.01\u2009g\u2009L<jats:sup>\u22121<\/jats:sup> d<jats:sup>\u22121<\/jats:sup>) and areal (20.3\u2009\u00b1\u20093.2\u2009g\u2009m<jats:sup>\u22122<\/jats:sup> d<jats:sup>\u22121<\/jats:sup>) biomass productivities were attained in the 100-m<jats:sup>3<\/jats:sup> PBR compared to those of the 35-m<jats:sup>3<\/jats:sup> PBR (0.05\u2009\u00b1\u20090.02\u2009g\u2009L<jats:sup>\u22121<\/jats:sup> d<jats:sup>\u22121<\/jats:sup> and 13.5\u2009\u00b1\u20094.3\u2009g\u2009m<jats:sup>\u22122<\/jats:sup> d<jats:sup>\u22121<\/jats:sup>, respectively). Lipid contents were similar in both PBRs (9\u201310% of ash free dry weight). CO<jats:sub>2<\/jats:sub> sequestration was followed in the 100-m<jats:sup>3<\/jats:sup> PBR, revealing a mean CO<jats:sub>2<\/jats:sub> mitigation efficiency of 65% and a biomass to carbon ratio of 1.80. <jats:italic>Tetraselmis<\/jats:italic> sp. CTP4 is thus a robust candidate for industrial-scale production with promising biomass productivities and photosynthetic efficiencies up to 3.5% of total solar irradiance.<\/jats:p>","DOI":"10.1038\/s41598-018-23340-3","type":"journal-article","created":{"date-parts":[[2018,3,29]],"date-time":"2018-03-29T01:29:27Z","timestamp":1522286967000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Scale-up and large-scale production of Tetraselmis sp. 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