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The present study aims to unravel the toxicity mechanisms of a widely used herbicide to the arthropod <jats:italic>Folsomia candida<\/jats:italic> exposed in a natural soil, by linking effects on reproduction, proteomics and genome-wide gene expression. The EC50 effects on reproduction over 4 weeks was 4.63\u2009mg glyphosate\/kg of soil. The formulation included a polyethoxylated tallowamine as an adjuvant, which at 50% effect on reproduction had an estimated concentration of 0.87\u20131.49\u2009mg\/kg of soil. No effects were observed on survival and reproduction when using the isolated active substance, pointing the toxicity of the formulated product to the co-formulant instead of the active ingredient, glyphosate. RNA sequencing and shotgun proteomics were applied to assess differential transcript and protein expressions between exposed and control organisms in time, respectively. Specific functional categories at protein and transcriptome levels were concordant with each other, despite overall limited correlations between datasets. The exposure to this formulation affected normal cellular respiration and lipid metabolism, inducing oxidative stress and leading to impairment in biological life cycle mechanisms such as molting and reproduction.<\/jats:p>","DOI":"10.1038\/s41598-018-29662-6","type":"journal-article","created":{"date-parts":[[2018,7,23]],"date-time":"2018-07-23T11:40:46Z","timestamp":1532346046000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide"],"prefix":"10.1038","volume":"8","author":[{"given":"Tiago","family":"Sim\u00f5es","sequence":"first","affiliation":[]},{"given":"Sara C.","family":"Novais","sequence":"additional","affiliation":[]},{"given":"Tiago","family":"Natal-da-Luz","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9764-2581","authenticated-orcid":false,"given":"Bart","family":"Devreese","sequence":"additional","affiliation":[]},{"given":"Tjalf","family":"de Boer","sequence":"additional","affiliation":[]},{"given":"Dick","family":"Roelofs","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9 P.","family":"Sousa","sequence":"additional","affiliation":[]},{"given":"Nico M.","family":"van Straalen","sequence":"additional","affiliation":[]},{"given":"Marco F. 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