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Despite all the progress in the development of potential anti-cancer antibodies, their application is frequently hindered by low specificities and cross-reactivity. In this study, a novel anti-STn monoclonal antibody named L2A5 was developed by hybridoma technology. Flow cytometry analysis showed that L2A5 specifically binds to sialylated structures on the cell surface of STn-expressing breast and bladder cancer cell lines. Moreover, immunoblotting assays demonstrated reactivity to tumour-associated <jats:italic>O<\/jats:italic>-glycosylated proteins, such as MUC1. Tumour recognition was further observed using immunohistochemistry assays, which demonstrated a high sensitivity and specificity of L2A5 mAb towards cancer tissue, using bladder and colorectal cancer tissues. L2A5 staining was exclusively tumoural, with a remarkable reactivity in invasive and metastasis sites, not detectable by other anti-STn mAbs. Additionally, it stained 20% of cases of triple-negative breast cancers, suggesting application in diseases with unmet clinical needs. Finally, the fine specificity was assessed using glycan microarrays, demonstrating a highly specific binding of L2A5 to core STn antigens and additional ability to bind 2\u20136-linked sialyl core-1 probes. In conclusion, this study describes a novel anti-STn antibody with a unique binding specificity that can be applied for cancer diagnostic and future development of new antibody-based therapeutic applications.<\/jats:p>","DOI":"10.1038\/s41598-018-30421-w","type":"journal-article","created":{"date-parts":[[2018,8,9]],"date-time":"2018-08-09T11:51:10Z","timestamp":1533815470000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Novel monoclonal antibody L2A5 specifically targeting sialyl-Tn and short glycans terminated by alpha-2\u20136 sialic acids"],"prefix":"10.1038","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7150-6354","authenticated-orcid":false,"given":"Liliana R.","family":"Loureiro","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3474-5417","authenticated-orcid":false,"given":"Diana P.","family":"Sousa","sequence":"additional","affiliation":[]},{"given":"Dylan","family":"Ferreira","sequence":"additional","affiliation":[]},{"given":"Wengang","family":"Chai","sequence":"additional","affiliation":[]},{"given":"Lu\u00eds","family":"Lima","sequence":"additional","affiliation":[]},{"given":"Carina","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"Carla B.","family":"Lopes","sequence":"additional","affiliation":[]},{"given":"Viviana G.","family":"Correia","sequence":"additional","affiliation":[]},{"given":"Lisete M.","family":"Silva","sequence":"additional","affiliation":[]},{"given":"Chunxia","family":"Li","sequence":"additional","affiliation":[]},{"given":"L\u00facio Lara","family":"Santos","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0097-6148","authenticated-orcid":false,"given":"Jos\u00e9 Alexandre","family":"Ferreira","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6245-8549","authenticated-orcid":false,"given":"Ana","family":"Barbas","sequence":"additional","affiliation":[]},{"given":"Angelina S.","family":"Palma","sequence":"additional","affiliation":[]},{"given":"Carlos","family":"Novo","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5987-2485","authenticated-orcid":false,"given":"Paula A.","family":"Videira","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2018,8,15]]},"reference":[{"key":"30421_CR1","doi-asserted-by":"publisher","first-page":"7","DOI":"10.3322\/caac.21387","volume":"67","author":"RL Siegel","year":"2017","unstructured":"Siegel, R. 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