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ZbHaa1 is the functional homologue of <jats:italic>S. cerevisiae<\/jats:italic> Haa1 and a bifunctional transcription factor able to modulate <jats:italic>Z. bailii<\/jats:italic> adaptive response to acetic acid and copper stress. In this study, RNA-Seq was used to investigate genomic transcription changes in <jats:italic>Z. bailii<\/jats:italic> during early response to sublethal concentrations of acetic acid (140\u2009mM, pH 4.0) or copper (0.08\u2009mM) and uncover the regulatory network activated by these stresses under ZbHaa1 control. Differentially expressed genes in response to acetic acid exposure (297) are mainly related with the tricarboxylic acid cycle, protein folding and stabilization and modulation of plasma membrane composition and cell wall architecture, 17 of which, directly or indirectly, ZbHaa1-dependent. Copper stress induced the differential expression of 190 genes mainly involved in the response to oxidative stress, 15 ZbHaa1-dependent. This study provides valuable mechanistic insights regarding <jats:italic>Z. bailii<\/jats:italic> adaptation to acetic acid or copper stress, as well as useful information on transcription regulatory networks in pre-whole genome duplication (WGD) (<jats:italic>Z. bailii<\/jats:italic>) and post-WGD (<jats:italic>S. cerevisiae<\/jats:italic>) yeast species, contributing to the understanding of transcriptional networks\u2019 evolution in yeasts.<\/jats:p>","DOI":"10.1038\/s41598-018-32266-9","type":"journal-article","created":{"date-parts":[[2018,9,14]],"date-time":"2018-09-14T11:19:20Z","timestamp":1536923960000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Transcriptional profiling of Zygosaccharomyces bailii early response to acetic acid or copper stress mediated by ZbHaa1"],"prefix":"10.1038","volume":"8","author":[{"given":"Miguel","family":"Antunes","sequence":"first","affiliation":[]},{"given":"Margarida","family":"Palma","sequence":"additional","affiliation":[]},{"given":"Isabel","family":"S\u00e1-Correia","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2018,9,20]]},"reference":[{"key":"32266_CR1","doi-asserted-by":"crossref","unstructured":"S\u00e1-Correia, I., Guerreiro, J. 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