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A mass spectrometry-based screening of potential cyanobactin producers led to the discovery of a new prenylated member of this family of compounds, sphaerocyclamide (1), from\n                    <jats:italic>Sphaerospermopsis<\/jats:italic>\n                    sp. LEGE 00249. The sphaerocyclamide biosynthetic gene cluster (\n                    <jats:italic>sph<\/jats:italic>\n                    ) encoding the novel macrocyclic prenylated cyanobactin, was sequenced. Heterologous expression of the\n                    <jats:italic>sph<\/jats:italic>\n                    gene cluster in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    confirmed the connection between genomic and mass spectrometric data. Unambiguous establishment of the orientation and site of prenylation required the full structural elucidation of 1 using Nuclear Magnetic Resonance (NMR), which demonstrated that a forward prenylation occurred on the tyrosine residue. Compound 1 was tested in pharmacologically or ecologically relevant biological assays and revealed moderate antimicrobial activity towards the fouling bacterium\n                    <jats:italic>Halomonas aquamarina<\/jats:italic>\n                    CECT 5000.\n                  <\/jats:p>","DOI":"10.1038\/s41598-018-32618-5","type":"journal-article","created":{"date-parts":[[2018,9,24]],"date-time":"2018-09-24T10:17:13Z","timestamp":1537784233000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Sphaerocyclamide, a prenylated cyanobactin from the cyanobacterium Sphaerospermopsis sp. 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