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They offer many advantages compared to antibiotics. The American Foulbrood (AFB) is a bacterial disease affecting honeybee larvae caused by<jats:italic>Paenibacillus larvae<\/jats:italic>. Phages can be very significant in fighting it mostly due to European restrictions to the use of antibiotics in beekeeping. New phages able to control<jats:italic>P. larvae<\/jats:italic>in hives have already been reported with satisfactory results. However, the efficacy and feasibility of administering phages indirectly to larvae through their adult workers only by providing phages in bees\u2019 feeders has never been evaluated. This strategy is considered herein the most feasible as far as hive management is concerned. This<jats:italic>in vivo<\/jats:italic>study investigated the ability of a phage to reach larvae in an infective state after oral administration to honeybees. The screening (by direct PFU count) and quantification (by quantitative PCR) of the phage in bee organs and in larvae after ingestion allowed us to conclude that despite 10<jats:sup>4<\/jats:sup>phages reaching larvae only an average of 32 were available to control the spread of the disease. The fast inactivation of many phages in royal jelly could compromise this therapeutic approach. The protection of phages from hive-derived conditions should be thus considered in further developments for AFB treatment.<\/jats:p>","DOI":"10.1038\/s41598-018-36432-x","type":"journal-article","created":{"date-parts":[[2019,1,24]],"date-time":"2019-01-24T11:04:17Z","timestamp":1548327857000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model"],"prefix":"10.1038","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4670-0753","authenticated-orcid":false,"given":"Henrique G.","family":"Ribeiro","sequence":"first","affiliation":[]},{"given":"Rossana","family":"Correia","sequence":"additional","affiliation":[]},{"given":"Tiago","family":"Moreira","sequence":"additional","affiliation":[]},{"given":"Diana","family":"Vilas Boas","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Azeredo","sequence":"additional","affiliation":[]},{"given":"Ana","family":"Oliveira","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2019,1,24]]},"reference":[{"key":"36432_CR1","doi-asserted-by":"publisher","first-page":"66","DOI":"10.4161\/bact.1.2.15845","volume":"1","author":"ST Abedon","year":"2011","unstructured":"Abedon, S. 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