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We show that high stress gradients, produced when picosecond laser pulses with a fluence of 100 mJ\/cm<jats:sup>2<\/jats:sup> are absorbed by piezophotonic materials, enable transfection of a plasmid DNA encoding Green Fluorescent Protein (gWizGFP, 3.74 MDa) in COS-7 monkey fibroblast cells with an efficiency of 5% at 20\u2009\u00b0C, in 10\u2009minutes. We did not observe significant cytotoxicity under these conditions. 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