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Our aim was to evaluate the expression of miR-16, miR-20a, miR-150 and miR-155 in several grades of ASIL obtained from high-risk patients, submitted to anal cancer screening from July 2016 to January 2017. Lesions were classified according to the Lower Anogenital Squamous Terminology (LAST) in low-grade (LSIL) and high-grade squamous intraepithelial lesions (HSIL), and the AIN classification in AIN1, AIN2 and AIN3. A hundred and five biopsies were obtained from 60 patients. Ten samples were negative (9.5%), 63 were LSIL (60%) and 32 were HSIL (30.5%) according to the LAST. Twenty seven (26%) were negative for dysplasia, 46 were classified as AIN1 (44%), 14 as AIN2 (13%) and 18 as AIN3 (17%) according to the AIN classification. There was no statistically significant difference in the fold expression of miR-16, miR-20a, miR-150 and miR-155, according to either classification. Although non- significant, there was an increasing trend in the miR-155 fold expression from negative samples to HSIL, with the highest fold expression increase in both LSIL and HSIL compared to the other miRNAs.<\/jats:p>","DOI":"10.1038\/s41598-018-38378-6","type":"journal-article","created":{"date-parts":[[2019,2,6]],"date-time":"2019-02-06T11:04:47Z","timestamp":1549451087000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Expression of microRNAs 16, 20a, 150 and 155 in anal squamous intraepithelial lesions from high-risk groups"],"prefix":"10.1038","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5258-2987","authenticated-orcid":false,"given":"Andreia","family":"Albuquerque","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9023-1109","authenticated-orcid":false,"given":"Mara","family":"Fernandes","sequence":"additional","affiliation":[]},{"given":"Oliver","family":"Stirrup","sequence":"additional","affiliation":[]},{"given":"Ana Lu\u00edsa","family":"Teixeira","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6268-524X","authenticated-orcid":false,"given":"Joana","family":"Santos","sequence":"additional","affiliation":[]},{"given":"Marta","family":"Rodrigues","sequence":"additional","affiliation":[]},{"given":"Elisabete","family":"Rios","sequence":"additional","affiliation":[]},{"given":"Guilherme","family":"Macedo","sequence":"additional","affiliation":[]},{"given":"Rui","family":"Medeiros","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2019,2,6]]},"reference":[{"key":"38378_CR1","doi-asserted-by":"publisher","first-page":"664","DOI":"10.1002\/ijc.30716","volume":"141","author":"C de Martel","year":"2017","unstructured":"de Martel, C., Plummer, M., Vignat, J. & Franceschi, S. 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