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Cells were characterized at gene, protein and functional levels. Functionally, both arterial and venous-like iPSC-derived ECs responded to vasoactive agonists such as thrombin and prostaglandin E2 (PGE<jats:sub>2<\/jats:sub>), similar to somatic ECs; however, arterial-like iPSC-derived ECs produced higher nitric oxide (NO) and elongation to shear stress than venous-like iPSC-derived ECs. Both cells adhered, proliferated and prevented platelet activation when seeded in poly(caprolactone) scaffolds. Interestingly, both iPSC-derived ECs cultured in monoculture or in a scaffold showed a different inflammatory profile than somatic ECs. Although both somatic and iPSC-derived ECs responded to tumor necrosis factor-\u03b1 (TNF-\u03b1) by an increase in the expression of intercellular adhesion molecule 1 (ICAM-1), only somatic ECs showed an upregulation in the expression of E-selectin or vascular cell adhesion molecule 1 (VCAM-1).<\/jats:p>","DOI":"10.1038\/s41598-019-40417-9","type":"journal-article","created":{"date-parts":[[2019,3,7]],"date-time":"2019-03-07T11:04:07Z","timestamp":1551956647000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":70,"title":["Functional characterization of iPSC-derived arterial- and venous-like endothelial cells"],"prefix":"10.1038","volume":"9","author":[{"given":"S.","family":"Rosa","sequence":"first","affiliation":[]},{"given":"C.","family":"Pra\u00e7a","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3116-0723","authenticated-orcid":false,"given":"P. 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