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This knowledge is particularly useful for phages infecting <jats:italic>Streptococcus thermophilus<\/jats:italic>, as they constitute a constant threat during dairy fermentations. Here, we explore the genetic diversity of <jats:italic>S. thermophilus<\/jats:italic> phages to identify genetic determinants with a signature for host specificity, which could be linked to the bacterial receptor genotype. A comparative genomic analysis was performed on 142\u00a0<jats:italic>S. thermophilus<\/jats:italic> phage genomes, 55 of which were sequenced in this study. Effectively, 94 phages were assigned to the group <jats:italic>cos<\/jats:italic> (DT1), 36 to the group <jats:italic>pac<\/jats:italic> (O1205), six to the group 5093, and six to the group 987. The core genome-based phylogeny of phages from the two dominating groups and their receptor binding protein (RBP) phylogeny corresponded to the phage host-range. A role of RBP in host recognition was confirmed by constructing a fluorescent derivative of the RBP of phage CHPC951, followed by studying the binding of the protein to the host strain. Furthermore, the RBP phylogeny of the <jats:italic>cos<\/jats:italic> group was found to correlate with the host genotype of the exocellular polysaccharide-encoding operon. These findings provide novel insights towards developing strategies to combat phage infections in dairies.<\/jats:p>","DOI":"10.1038\/s41598-019-44481-z","type":"journal-article","created":{"date-parts":[[2019,5,29]],"date-time":"2019-05-29T10:02:24Z","timestamp":1559124144000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["A comparative genomics approach for identifying host-range determinants in Streptococcus thermophilus bacteriophages"],"prefix":"10.1038","volume":"9","author":[{"given":"Paula","family":"Szymczak","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5098-3453","authenticated-orcid":false,"given":"Martin Holm","family":"Rau","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o M.","family":"Monteiro","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7132-8842","authenticated-orcid":false,"given":"Mariana G.","family":"Pinho","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4485-832X","authenticated-orcid":false,"given":"S\u00e9rgio Raposo","family":"Filipe","sequence":"additional","affiliation":[]},{"given":"Finn Kvist","family":"Vogensen","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9984-5625","authenticated-orcid":false,"given":"Ahmad A.","family":"Zeidan","sequence":"additional","affiliation":[]},{"given":"Thomas","family":"Janzen","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2019,5,29]]},"reference":[{"key":"44481_CR1","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/j.copbio.2014.09.006","volume":"32","author":"J Mahony","year":"2015","unstructured":"Mahony, J. & van Sinderen, D. 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