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In this work, a <jats:italic>de novo<\/jats:italic> designed antimicrobial peptide (AMP) Chain201D was studied in the context of urinary catheter-associated infections. Chain201D showed excellent antimicrobial activity against relevant ATCC strains and clinical isolates of bacteria and yeast and demonstrated high stability in a wide range of temperatures, pH and salt concentrations. Moreover, the bactericidal activity of Chain201D immobilized on a model surface was studied against <jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic>) and <jats:italic>Staphylococcus aureus<\/jats:italic> (<jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic>), some of the most prevalent strains found in urinary catheter-associated infections. Chain201D was successfully tethered to ((1-mercapto-11-undecyl)-(tetra(ethylene glycol) (EG4)) terminated self-assembled monolayers (SAMs), (EG4-SAMs), activated by 1,1\u2032-Carbonyldiimidazole (CDI) at different concentrations. Chain201D surfaces can bind and kill by contact a high percentage of adherent bacteria. These achievements are obtained without any peptide modification (for chemoselective conjugation) and without the use of a spacer. Moreover, increased amounts of immobilized AMP lead to higher numbers of adhered\/dead bacteria, revealing a concentration-dependent behaviour and demonstrating that Chain201D has excellent potential for developing antimicrobial urinary catheters.<\/jats:p>","DOI":"10.1038\/s41598-019-47108-5","type":"journal-article","created":{"date-parts":[[2019,7,24]],"date-time":"2019-07-24T13:07:35Z","timestamp":1563973655000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Prevention of urinary catheter-associated infections by coating antimicrobial peptides from crowberry endophytes"],"prefix":"10.1038","volume":"9","author":[{"given":"Claudia","family":"Monteiro","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8981-7049","authenticated-orcid":false,"given":"Fab\u00edola","family":"Costa","sequence":"additional","affiliation":[]},{"given":"Anna Maria","family":"Pirttil\u00e4","sequence":"additional","affiliation":[]},{"given":"Mysore V.","family":"Tejesvi","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6574-4794","authenticated-orcid":false,"given":"M. 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