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API480 encodes 77 coding sequences (CDSs) on its 45,026\u2009bp dsDNA genome, of which 47 were confirmed using mass spectrometry. This phage has got very limited genomic and proteomic similarity to any other known ones registered in public databases, including <jats:italic>P. larvae<\/jats:italic> phages. Comparative genomics indicates API480 is a new species as it\u2019s a singleton with 28 unique proteins. Interestingly, the lysis module is highly conserved among <jats:italic>P. larvae<\/jats:italic> phages, containing a predicted endolysin and two putative holins. The well kept overall genomic organisation (from the structural and morphogenetic modules to the host lysis, DNA replication and metabolism related proteins) confirms a common evolutionary ancestor among <jats:italic>P. larvae<\/jats:italic> infecting phages. API480 is able to infect 69% of the 61 field strains with an ERIC I genotype, as well as ERIC II strains. 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