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Since the structure of briarane B-3 (<jats:bold>1<\/jats:bold>) was only suggested and published without any spectroscopic support, it was herein confirmed, and the supporting data are now provided. In addition, <jats:bold>1<\/jats:bold> provided the opportunity to explore the sensitivity of vibrational circular dichroism (VCD) to determine the configuration of a single stereogenic center in the presence of eight other stereogenic centers in a molecule possessing a highly flexible ten-member ring. A single-crystal X-ray diffraction study, in which the Flack and Hooft parameters of <jats:bold>1<\/jats:bold> were determined, further confirmed that briarane B-3 is (1<jats:italic>S<\/jats:italic>,2<jats:italic>S<\/jats:italic>,6<jats:italic>S<\/jats:italic>,7<jats:italic>R<\/jats:italic>,8<jats:italic>R<\/jats:italic>,9<jats:italic>S<\/jats:italic>,10<jats:italic>S<\/jats:italic>,11<jats:italic>R<\/jats:italic>,17<jats:italic>R<\/jats:italic>)-<jats:bold>1<\/jats:bold>. This paper reports for first time the use of VCD in briarane diterpenes and with the presence of chlorine atoms. Biological evaluation of seven isolated compounds evidenced a moderate anti-inflammatory activity for compounds <jats:bold>6<\/jats:bold> and <jats:bold>9<\/jats:bold> but it did not show any cytotoxic, antiviral, antibacterial, and topoisomerase inhibitory activity.<\/jats:p>","DOI":"10.1038\/s41598-020-79774-1","type":"journal-article","created":{"date-parts":[[2021,1,12]],"date-time":"2021-01-12T18:36:08Z","timestamp":1610476568000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Absolute configuration by vibrational circular dichroism of anti-inflammatory macrolide briarane diterpenoids from the Gorgonian Briareum asbestinum"],"prefix":"10.1038","volume":"11","author":[{"given":"Dawrin","family":"Pech-Puch","sequence":"first","affiliation":[]},{"given":"Pedro","family":"Joseph-Nathan","sequence":"additional","affiliation":[]},{"given":"Eleuterio","family":"Burgue\u00f1o-Tapia","sequence":"additional","affiliation":[]},{"given":"Carlos","family":"Gonz\u00e1lez-Salas","sequence":"additional","affiliation":[]},{"given":"Diana","family":"Mart\u00ednez-Matamoros","sequence":"additional","affiliation":[]},{"given":"David M.","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"Renato B.","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"Carlos","family":"Jim\u00e9nez","sequence":"additional","affiliation":[]},{"given":"Jaime","family":"Rodr\u00edguez","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2021,1,12]]},"reference":[{"key":"79774_CR1","doi-asserted-by":"publisher","first-page":"15964","DOI":"10.1002\/anie.201905312","volume":"58","author":"JS Dickschat","year":"2019","unstructured":"Dickschat, J. 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