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In aquaculture, a current challenge is to feed carnivorous fish with plant-feedstuffs in substitution of fish meal, an unsustainable commodity. Plants have a limited nutritive value due to the presence of non-starch polysaccharides (NSP) which are not metabolized by fish. In this work we assessed the effects of NSP-enriched diets on European seabass gut microbiota and evaluate the selective pressure of plant feedstuffs towards gut microbes with NSP-hydrolytic potential, i.e. capable to convert indigestible dietary constituents in fish metabolites. Triplicate groups of European seabass juveniles were fed a fish meal-based diet (control) or three plant-based diets (SBM, soybean meal; RSM, rapeseed meal; SFM, sunflower meal) for 6\u00a0weeks, before recovering intestinal samples for microbiota analysis, using the Illumina\u2019s MiSeq platform. Plant-based diets impacted differently digesta and mucosal microbiota. A decrease (<jats:italic>p<\/jats:italic>\u2009=\u20090.020) on species richness, accompanied by a decline on the relative abundance of specific phyla such as Acidobacteria (<jats:italic>p<\/jats:italic>\u2009=\u20090.030), was observed in digesta samples of SBM and RSM experimental fish, but no effects were seen in mucosa-associated microbiota. Plant-based diets favored the Firmicutes (<jats:italic>p<\/jats:italic>\u2009=\u20090.01), in particular the Bacillaceae (<jats:italic>p<\/jats:italic>\u2009=\u20090.017) and Clostridiaceae (<jats:italic>p<\/jats:italic>\u2009=\u20090.007), two bacterial families known to harbor carbohydrate active enzymes and thus putatively more prone to grow in high NSP environments. Overall, bacterial gut communities of European seabass respond to plant-feedstuffs with adjustments in the presence of transient microorganisms (allochthonous) with carbohydrolytic potential, while maintaining a balanced core (autochthonous) microbiota.<\/jats:p>","DOI":"10.1038\/s41598-020-80138-y","type":"journal-article","created":{"date-parts":[[2021,1,11]],"date-time":"2021-01-11T11:49:18Z","timestamp":1610365758000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Gut microbiota dynamics in carnivorous European seabass (Dicentrarchus labrax) fed plant-based diets"],"prefix":"10.1038","volume":"11","author":[{"given":"Cl\u00e1udia R.","family":"Serra","sequence":"first","affiliation":[]},{"given":"Aires","family":"Oliva-Teles","sequence":"additional","affiliation":[]},{"given":"Paula","family":"Enes","sequence":"additional","affiliation":[]},{"given":"Fernando","family":"Tavares","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2021,1,11]]},"reference":[{"key":"80138_CR1","doi-asserted-by":"publisher","first-page":"1372","DOI":"10.1093\/ajcn\/25.12.1372","volume":"25","author":"DC Savage","year":"1972","unstructured":"Savage, D. 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