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Here we show that photoacoustic waves can promote the release of FITC-dextran or GFP from GUVs without damage. Real-time interferometric imaging offers the first movies of photoacoustic wave propagation and interaction with GUVs. The photoacoustic waves are seen as mostly compressive half-cycle pulses with peak pressures of\u2009~\u20091\u00a0MPa and spatial extent FWHM\u2009~\u200936\u00a0\u00b5m. At a repetition rate of 10\u00a0Hz, they enable the release of 25% of the FITC-dextran content of GUVs in 15\u00a0min. Such photoacoustic waves may enable non-invasive targeted release of GUVs and cell transfection over large volumes of tissues in just a few minutes.<\/jats:p>","DOI":"10.1038\/s41598-021-82140-4","type":"journal-article","created":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T13:21:09Z","timestamp":1612272069000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Imaging of photoacoustic-mediated permeabilization of giant unilamellar vesicles (GUVs)"],"prefix":"10.1038","volume":"11","author":[{"given":"Diogo A.","family":"Pereira","sequence":"first","affiliation":[]},{"given":"Alexandre D.","family":"Silva","sequence":"additional","affiliation":[]},{"given":"Patricia A. 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