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The pentose sugar<jats:sc>d<\/jats:sc>-xylose is often present in significant amounts along with hexoses.<jats:italic>Saccharomyces cerevisiae<\/jats:italic>can acquire the ability to metabolize<jats:sc>d<\/jats:sc>-xylose through expression of heterologous<jats:sc>d<\/jats:sc>-xylose isomerase (XI). This enzyme is notoriously difficult to express in<jats:italic>S. cerevisiae<\/jats:italic>and only fourteen XIs have been reported to be active so far. We cloned a new<jats:sc>d<\/jats:sc>-xylose isomerase derived from microorganisms in the gut of the wood-feeding beetle<jats:italic>Odontotaenius disjunctus<\/jats:italic>. Although somewhat homologous to the XI from<jats:italic>Piromyces<\/jats:italic>sp. E2, the new gene was identified as bacterial in origin and the host as a<jats:italic>Parabacteroides<\/jats:italic>sp. Expression of the new XI in<jats:italic>S. cerevisiae<\/jats:italic>resulted in faster aerobic growth than the XI from<jats:italic>Piromyces<\/jats:italic>on<jats:sc>d<\/jats:sc>-xylose media. The<jats:sc>d<\/jats:sc>-xylose isomerization rate conferred by the new XI was also 72% higher, while absolute xylitol production was identical in both strains. Interestingly, increasing concentrations of xylitol (up to 8\u00a0g\u00a0L<jats:sup>\u22121<\/jats:sup>) appeared not to inhibit<jats:sc>d<\/jats:sc>-xylose consumption. The newly described XI displayed 2.6 times higher specific activity, 37% lower K<jats:sub>M<\/jats:sub>for<jats:sc>d<\/jats:sc>-xylose, and exhibited higher activity over a broader temperature range, retaining 51% of maximal activity at 30\u00a0\u00b0C compared with only 29% activity for the<jats:italic>Piromyces<\/jats:italic>XI.<\/jats:p>","DOI":"10.1038\/s41598-021-83937-z","type":"journal-article","created":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T11:03:35Z","timestamp":1614337415000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["A novel d-xylose isomerase from the gut of the wood feeding beetle Odontotaenius disjunctus efficiently expressed in Saccharomyces cerevisiae"],"prefix":"10.1038","volume":"11","author":[{"given":"Paulo C\u00e9sar","family":"Silva","sequence":"first","affiliation":[]},{"given":"Javier A.","family":"Ceja-Navarro","sequence":"additional","affiliation":[]},{"given":"Fl\u00e1vio","family":"Azevedo","sequence":"additional","affiliation":[]},{"given":"Ulas","family":"Karaoz","sequence":"additional","affiliation":[]},{"given":"Eoin L.","family":"Brodie","sequence":"additional","affiliation":[]},{"given":"Bj\u00f6rn","family":"Johansson","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2021,2,26]]},"reference":[{"key":"83937_CR1","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1016\/j.ijbiomac.2017.02.097","volume":"99","author":"A Arevalo-Gallegos","year":"2017","unstructured":"Arevalo-Gallegos, A., Ahmad, Z., Asgher, M., Parra-Saldivar, R. & Iqbal, H. 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