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A previously characterized individual alginate lyase, belonging to the family 7 of polysaccharide lyases (PL7) and produced by <jats:italic>Saccharophagus degradans<\/jats:italic>, was shown to be the most efficient in the in vitro degradation of <jats:italic>L. digitata<\/jats:italic> cell wall. The alginate lyase treatment, compared to the control, released up to 7.11\u00a0g\/L of reducing sugars (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and 8.59\u00a0mmol\/100\u00a0g dried alga of monosaccharides (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), and reduced cell wall fluorescence intensity by 39.1% after staining with Calcofluor White (<jats:italic>p<\/jats:italic>\u2009=\u20090.001). The hydrolysis of gel-forming polymer alginate by the alginate lyase treatment could prevent the trapping of fatty acids and release beneficial monounsaturated fatty acids, particularly 18:1<jats:italic>c<\/jats:italic>9 (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), to the extracellular medium. However, no liberation of proteins (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.170) or pigments (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.070) was observed. Overall, these results show the ability of an individual alginate lyase, from PL7 family, to partially degrade <jats:italic>L. digitata<\/jats:italic> cell wall under physiological conditions. Therefore, this CAZyme can potentially improve the bioavailability of <jats:italic>L. digitata<\/jats:italic> bioactive compounds for monogastric diets, with further application in feed industry.<\/jats:p>","DOI":"10.1038\/s41598-021-89278-1","type":"journal-article","created":{"date-parts":[[2021,5,6]],"date-time":"2021-05-06T10:04:35Z","timestamp":1620295475000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall"],"prefix":"10.1038","volume":"11","author":[{"given":"M\u00f3nica","family":"Costa","sequence":"first","affiliation":[]},{"given":"Lu\u00eds","family":"Pio","sequence":"additional","affiliation":[]},{"given":"Pedro","family":"Bule","sequence":"additional","affiliation":[]},{"given":"V\u00e2nia","family":"Cardoso","sequence":"additional","affiliation":[]},{"given":"Cristina M.","family":"Alfaia","sequence":"additional","affiliation":[]},{"given":"Diogo","family":"Coelho","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Br\u00e1s","sequence":"additional","affiliation":[]},{"given":"Carlos M. 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