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The preparations of this enzyme for clinical use are derived from bacterial sources and its use is associated with serious adverse reactions. In this context, it is important to find new sources of L-ASNase. In this work, the Placket-Burman Experimental Design (PBD) was used to determine the influence of the variables on the L-ASNase production then it was followed by a 2<jats:sup>8\u20134<\/jats:sup> Factorial Fractional Design (FFD). The results obtained from PBD have shown a range of L-ASNase activity, from 0.47 to 1.77\u00a0U\/gcell and the results obtained from FFD have showed a range of L-ASNase activity, from 1.10 to 2.36 U\/gcell. L-proline and ammonium sulfate were identified as of significant positive variables on this production enzyme by <jats:italic>Penicillium cerradense<\/jats:italic> sp. nov. The precise identification of this new species was confirmed by morphological characteristics and sequence comparisons of the nuclear 18S-5.8S-28S partial nrDNA including the ITS1 and ITS2 regions, RNA polymerase II, \u03b2-tubulin and calmodulin genomic regions. The genetic sequence coding for the L-ASNase was obtained after carrying out a full genome sequencing. The L-ASNase expressed by <jats:italic>P. cerradense<\/jats:italic> sp. nov may have promising antineoplastic properties.<\/jats:p>","DOI":"10.1038\/s41598-021-97316-1","type":"journal-article","created":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T10:06:24Z","timestamp":1631181984000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Sequencing and characterization of an L-asparaginase gene from a new species of Penicillium section Citrina isolated from Cerrado"],"prefix":"10.1038","volume":"11","author":[{"given":"Kellen C. 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