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We have achieved this goal by preparing a co-polymer of the commercial <jats:italic>N<\/jats:italic>-vinyl-2-pyrrolidone (99.5% mol) and a fluorescent substrate for trypsin activity determination\u00a0having a vinylic group (0.5%). The activity of trypsin has been measured in water solutions of this polymer over time, contrasted against the activity of both the commercial substrate Z-L-Arg-7-amido-4-methylcoumarin hydrochloride and its monomeric derivative, prepared <jats:italic>ad-hoc<\/jats:italic>. Initially, the activity of the sensory polymer was 74.53\u2009\u00b1\u20091.72\u00a0nmol\/min\/mg of enzyme, while that of the commercial substrate was 20.44\u2009\u00b1\u20090.65\u00a0nmol\/min\/mg of enzyme, the former maintained stable along weeks and the latter with a deep decay to zero in three weeks. The \u2018protection\u2019 effect exerted by the polymer chain has been studied by solvation studies by UV\u2013Vis spectroscopy, steady-state &amp; time resolved fluorescence, thermogravimetry and isothermal titration calorimetry.<\/jats:p>","DOI":"10.1038\/s41598-022-12848-4","type":"journal-article","created":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T09:04:44Z","timestamp":1653469484000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The role of polymeric chains as a protective environment for improving the stability and efficiency of fluorogenic peptide substrates"],"prefix":"10.1038","volume":"12","author":[{"given":"Ana","family":"Arnaiz","sequence":"first","affiliation":[]},{"given":"Marta","family":"Guembe-Garc\u00eda","sequence":"additional","affiliation":[]},{"given":"Estefan\u00eda","family":"Delgado-Pinar","sequence":"additional","affiliation":[]},{"given":"Artur J. 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