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SDS\u2013Polyacrylamide Gel Electrophoresis (SDS-PAGE) protein profile and a panel of 25 specific biomarkers of oxidative status, stress, metabolism and tissue damage were evaluated in samples subjected to different treatments: thermal (65 \u00b0C or 92 \u00b0C) and chemical with detergents [sodium dodecyl sulphate (SDS), Triton X-100 or NP-40]. Salivary SDS-PAGE profile was most affected by heating at 92 \u00b0C, with three and two protein bands decreasing and increasing their expression levels, respectively. This treatment also affected the results of several enzymes, with some of them being also affected by heating at 65 \u00b0C and incubation with SDS. The use of Triton X-100 or NP-40 resulted in  increased values of cortisol, triglycerides and glucose, not affecting the other tested biomarkers. The present results will help researchers and clinicians to select the best protocols to work in safe conditions with saliva, taking into account the target analyte planned to be measured.<\/jats:p>","DOI":"10.1038\/s41598-022-13491-9","type":"journal-article","created":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T09:06:14Z","timestamp":1654679174000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytes"],"prefix":"10.1038","volume":"12","author":[{"given":"Elsa","family":"Lamy","sequence":"first","affiliation":[]},{"given":"Camila P.","family":"Rubio","sequence":"additional","affiliation":[]},{"given":"Laura","family":"Carreira","sequence":"additional","affiliation":[]},{"given":"Fernando","family":"Capela e Silva","sequence":"additional","affiliation":[]},{"given":"Silvia","family":"Martinez-Subiela","sequence":"additional","affiliation":[]},{"given":"Fernando","family":"Tecles","sequence":"additional","affiliation":[]},{"given":"Pia","family":"Lopez-Jornet","sequence":"additional","affiliation":[]},{"given":"Jose J.","family":"Ceron","sequence":"additional","affiliation":[]},{"given":"Asta","family":"Tvarijonaviciute","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2022,6,8]]},"reference":[{"key":"13491_CR1","doi-asserted-by":"publisher","unstructured":"Tvarijonaviciute, A., Martinez-Subiela, S., Lopez-Jornet, P. & Lamy, E. 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