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This work aimed to investigate the correlation between STRs\u2019 specific repetitive motif composition and mutational dynamics, specifically the occurrence of single- or multistep mutations. Allelic transmission data, comprising 323,818 allele transfers and 1,297 mutations, were gathered for 35 Y-chromosomal STRs with simple structure. Six structure groups were established: ATT, CTT, TCTA\/GATA, GAAA\/CTTT, CTTTT, and AGAGAT, according to the repetitive motif present in the DNA leading strand of the markers. Results show that the occurrence of multistep mutations varies significantly among groups of markers defined by the repetitive motif. The group of markers with the highest frequency of multistep mutations was the one with repetitive motif CTTTT (25% of the detected mutations) and the lowest frequency corresponding to the group with repetitive motifs TCTA\/GATA (0.93%). Statistically significant differences (\u03b1\u2009=\u20090.05) were found between groups with repetitive motifs with different lengths, as is the case of TCTA\/GATA and ATT (p\u2009=\u20090.0168), CTT (p\u2009&lt;\u20090.0001) and CTTTT (p\u2009&lt;\u20090.0001), as well as between GAAA\/CTTT and CTTTT (p\u2009=\u20090.0102). The same occurred between the two tetrameric groups GAAA\/CTTT and TCTA\/GATA (p\u2009&lt;\u20090.0001) \u2013 the first showing 5.7 times more multistep mutations than the second. When considering the number of repeats of the mutated paternal alleles, statistically significant differences were found for alleles with 10 or 12 repeats, between GATA and ATT structure groups. These results, which demonstrate the heterogeneity of mutational dynamics across repeat motifs, have implications in the fields of population genetics, epidemiology, or phylogeography, and whenever STR mutation models are used in evolutionary studies in general.<\/jats:p>","DOI":"10.1038\/s41598-023-32137-y","type":"journal-article","created":{"date-parts":[[2023,6,24]],"date-time":"2023-06-24T10:05:47Z","timestamp":1687601147000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The sequence of the repetitive motif influences the frequency of multistep mutations in Short Tandem Repeats"],"prefix":"10.1038","volume":"13","author":[{"given":"Sofia","family":"Ant\u00e3o-Sousa","sequence":"first","affiliation":[]},{"given":"N\u00e1dia","family":"Pinto","sequence":"additional","affiliation":[]},{"given":"Pablo","family":"Rende","sequence":"additional","affiliation":[]},{"given":"Ant\u00f3nio","family":"Amorim","sequence":"additional","affiliation":[]},{"given":"Leonor","family":"Gusm\u00e3o","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,6,24]]},"reference":[{"issue":"6","key":"32137_CR1","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1038\/nrg1348","volume":"5","author":"H Ellegren","year":"2004","unstructured":"Ellegren, H. 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