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However, carbohydrate-active enzymes (CAZymes) such as Rovabio<jats:sup>\u00ae<\/jats:sup> Excel AP and alginate lyase can help break down these polysaccharides and render intracellular nutrients more accessible. This study aimed to evaluate the impact of 10% <jats:italic>L. digitata<\/jats:italic> feed inclusion and CAZyme supplementation on piglet blood cells, serum metabolites, liver lipid and mineral profiles. Forty weaned piglets were randomly assigned to one of four diets (n\u2009=\u200910 each): a control diet, 10% <jats:italic>L. digitata<\/jats:italic> (LA), 10% <jats:italic>L. digitata<\/jats:italic>\u2009+\u20090.005% Rovabio<jats:sup>\u00ae<\/jats:sup> Excel AP (LAR), and 10% <jats:italic>L. digitata<\/jats:italic>\u2009+\u20090.01% alginate lyase (LAL). After two weeks of trial, animals were slaughtered and liver and blood serum samples taken for analysis. The results showed that the LA and LAL diets increased blood lymphocytes, IgG and IgM, and decreased serum lipids, improving both cellular and humoral immune response and cardiovascular health. Dietary CAZymes reversed the anti-inflammatory and hematopoietic effects. Additionally, cortisol levels were reduced with seaweed inclusion compared to the control diet (P\u2009&lt;\u20090.001). In the liver, total n-3 PUFA and n-6\/n-3 ratio were increased and decreased, respectively, due to eicosapentaenoic acid and \u03b1-linolenic acid accumulation (P\u2009&lt;\u20090.001). However, total liver mineral content was incorporated to a lesser extent with the combined seaweed and enzyme diets (P\u2009&lt;\u20090.001), potentially indicating a negative effect on mineral bioavailability. Overall, results suggest that a 10% <jats:italic>L. digitata<\/jats:italic> inclusion can effectively improve piglet health by reducing stress during weaning, without the need for dietary CAZymes.<\/jats:p>","DOI":"10.1038\/s41598-023-33835-3","type":"journal-article","created":{"date-parts":[[2023,4,22]],"date-time":"2023-04-22T09:02:19Z","timestamp":1682154139000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Effect of Laminaria digitata dietary inclusion and CAZyme supplementation on blood cells, serum metabolites and hepatic lipids and minerals of weaned piglets"],"prefix":"10.1038","volume":"13","author":[{"given":"David M.","family":"Ribeiro","sequence":"first","affiliation":[]},{"given":"Rui M. 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