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Egg-laying harbours a great potential as a paradigm to uncover how the appropriate motor circuits are organized and activated to generate behaviour. To study this programme, we first describe the different phases of the egg-laying programme and the specific actions associated with each phase. Using a combination of neuronal activation and silencing experiments, we identify neurons (OvAbg) in the abdominal ganglion as key players in egg-laying. To generate and functionally characterise subsets of OvAbg, we used an intersectional approach with neurotransmitter specific lines\u2014\n                    <jats:italic>VGlut<\/jats:italic>\n                    ,\n                    <jats:italic>Cha<\/jats:italic>\n                    and\n                    <jats:italic>Gad1<\/jats:italic>\n                    . We show that OvAbg\/VGlut neurons promote initiation of egg deposition in a mating status dependent way. OvAbg\/Cha neurons are required in exploration and egg deposition phases, though activation leads specifically to egg expulsion. Experiments with the OvAbg\/Gad1 neurons show they participate in egg deposition. We further show a functional connection of OvAbg neurons with brain neurons. This study provides insight into the organization of neuronal circuits underlying complex motor behaviour.\n                  <\/jats:p>","DOI":"10.1038\/s41598-023-48109-1","type":"journal-article","created":{"date-parts":[[2023,12,11]],"date-time":"2023-12-11T08:02:33Z","timestamp":1702281753000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Neuronal substrates of egg-laying behaviour at the abdominal ganglion of Drosophila melanogaster"],"prefix":"10.1038","volume":"13","author":[{"given":"Cristina","family":"Oliveira-Ferreira","sequence":"first","affiliation":[]},{"given":"Miguel","family":"Gaspar","sequence":"additional","affiliation":[]},{"given":"Maria Lu\u00edsa","family":"Vasconcelos","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,12,11]]},"reference":[{"key":"48109_CR1","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1007\/BF00348055","volume":"36","author":"Y Cohet","year":"1978","unstructured":"Cohet, Y. & David, J. 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