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This study aims to evaluate the impact on the evidence\u2019s weighing, when different analytical threshold values are used, and when different models and\/or estimates for analytical artifacts, such as stutters or drop-in parameters, are considered. To reach this goal, three PGS, based on different statistical models, were used to analyze real casework pairs of samples composed of a mixture with either two or three estimated contributors, and a single-source sample associated. The obtained results show that the estimation of these parameters must not be overlooked, as they may considerably impact the outcome. This underlines the importance of proper parametrization in the analysis of forensic genetics identification problems when using complex samples, and the understanding by practitioners of how probabilistic genotyping informatics tools work to use them accurately.<\/jats:p>","DOI":"10.1038\/s41598-024-83841-2","type":"journal-article","created":{"date-parts":[[2025,1,20]],"date-time":"2025-01-20T12:57:03Z","timestamp":1737377823000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The impact of parameter variation in the quantification of forensic genetic evidence"],"prefix":"10.1038","volume":"15","author":[{"given":"Camila","family":"Costa","sequence":"first","affiliation":[]},{"given":"Carolina","family":"Figueiredo","sequence":"additional","affiliation":[]},{"given":"Sandra","family":"Costa","sequence":"additional","affiliation":[]},{"given":"Paulo Miguel","family":"Ferreira","sequence":"additional","affiliation":[]},{"given":"Ant\u00f3nio","family":"Amorim","sequence":"additional","affiliation":[]},{"given":"Lourdes","family":"Prieto","sequence":"additional","affiliation":[]},{"given":"N\u00e1dia","family":"Pinto","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2025,1,20]]},"reference":[{"issue":"4","key":"83841_CR1","doi-asserted-by":"publisher","first-page":"1004","DOI":"10.2144\/96206st01","volume":"20","author":"M Brownstein","year":"1996","unstructured":"Brownstein, M., Carpten, J. & Smith, J. 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