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Inadequate levels of maternally derived THs (MTH) supply affect target gene expression profiles, which are fundamental for the brain\u2019s normal growth, maturation, and function. The monocarboxylate transporter 8 (SLC16A2, MCT8) is the main TH transporter present in the brain during embryonic development, and mutations in this transporter lead to a rare and debilitating human condition known as the Allan-Herndon-Dudley Syndrome (AHDS). This mutation affects the capacity for intracellular transport of the hormone, leading to impaired brain development that constitutes the main pathophysiological basis of AHDS. Like humans, zebrafish embryos express <jats:italic>slc16a2<\/jats:italic> that transports exclusively T3 at zebrafish physiological temperature. Studies in zebrafish Mct8 knockdown (KD) models found impaired hindbrain vasculature development. Here, using zebrafish Mct8 KD and knockout (KO) models, we shed light on the maternal T3 (MT3)-dependent developmental mechanism behind hindbrain vasculature development. We first demonstrate that MT3-regulates hindbrain <jats:italic>vegfaa<\/jats:italic> expression. We provide evidence that hindbrain neurons are not the source of <jats:italic>vegfaa<\/jats:italic>, instead, restricted <jats:italic>pax6a+<\/jats:italic> neuroprogenitor cells (NPCs) instruct central arteries (CtAs) ingression into the hindbrain. Therefore, MT3 acts as an integrator, providing the regulatory cues necessary for the timely ingression of the CtAs into the hindbrain.<\/jats:p>","DOI":"10.1038\/s42003-025-08404-1","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T12:17:25Z","timestamp":1751372245000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Maternal thyroid hormone is required to develop the hindbrain vasculature in zebrafish"],"prefix":"10.1038","volume":"8","author":[{"given":"Marlene","family":"Trindade","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9881-4421","authenticated-orcid":false,"given":"N\u00e1dia","family":"Silva","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5767-4351","authenticated-orcid":false,"given":"Joana","family":"Rodrigues","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9993-1435","authenticated-orcid":false,"given":"Koichi","family":"Kawakami","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5238-0506","authenticated-orcid":false,"given":"Marco A.","family":"Campinho","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2025,7,1]]},"reference":[{"key":"8404_CR1","unstructured":"Bernal, J. 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