{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T09:37:11Z","timestamp":1769074631128,"version":"3.49.0"},"reference-count":67,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T00:00:00Z","timestamp":1753142400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T00:00:00Z","timestamp":1753142400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Ministry of Education and Science | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["2022.01066.PTDC"],"award-info":[{"award-number":["2022.01066.PTDC"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Ministry of Education and Science | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["FCT\/UIDPB\/04950\/2025"],"award-info":[{"award-number":["FCT\/UIDPB\/04950\/2025"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Commun Biol"],"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>Kisspeptins (Kiss) are key regulators of the hypothalamic-pituitary-gonadal axis, influencing testosterone surges essential for brain masculinization and behavioral development in mammals. This study explored the effects of transient neonatal Kiss blockade on long-term social behaviors in Wistar rats. Newborn rats of both sexes were injected with either a Kiss antagonist or vehicle during the postnatal testosterone surge, termed \u201cminipuberty\u201d. In adolescence and adulthood, social behaviors, hypothalamic Kiss receptor levels, and serum levels of Gonadotropin-releasing hormone (GnRH), luteinizing hormone (LH), testosterone, and follicle-stimulating hormone (FSH) were assessed. Results showed that neonatal Kiss modulates testosterone differently in males and females, influencing social communication and long-term social skills. Increased exploratory behavior was observed, with males exhibiting heightened sexual impulsiveness without anxiety changes. Altered hypothalamic-pituitary-gonadal hormone levels due to Kiss blockade may help explain some results. These findings highlight the critical role of neonatal Kiss in shaping lifelong social interactions and communication in a sex-dependent manner.<\/jats:p>","DOI":"10.1038\/s42003-025-08478-x","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T06:25:58Z","timestamp":1753165558000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The role of neonatal kisspeptin in long-term social behavior in mammals"],"prefix":"10.1038","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5854-8664","authenticated-orcid":false,"given":"Jo\u00e3o","family":"R. Neves","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0817-9917","authenticated-orcid":false,"given":"Miguel","family":"Castelo-Branco","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4364-6373","authenticated-orcid":false,"given":"Joana","family":"Gon\u00e7alves","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2025,7,22]]},"reference":[{"key":"8478_CR1","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1677\/JOE-07-0454","volume":"196","author":"RA Silvestre","year":"2008","unstructured":"Silvestre, R. A., Egido, E. M., Hern\u00e1ndez, R. & Marco, J. Kisspeptin-13 inhibits insulin secretion without affecting glucagon or somatostatin release: study in the perfused rat pancreas. J. Endocrinol. 196, 283\u2013290 (2008).","journal-title":"J. Endocrinol."},{"key":"8478_CR2","doi-asserted-by":"publisher","first-page":"1079","DOI":"10.1113\/JP281712","volume":"600","author":"C Izzi-Engbeaya","year":"2022","unstructured":"Izzi-Engbeaya, C. & Dhillo, W. S. Emerging roles for kisspeptin in metabolism. J. Physiol. 600, 1079\u20131088 (2022).","journal-title":"J. Physiol."},{"key":"8478_CR3","doi-asserted-by":"crossref","unstructured":"Zhang, P. et al. Expression and function of kisspeptin during mouse decidualization. PLoS ONE 9, e97647 (2014).","DOI":"10.1371\/journal.pone.0097647"},{"key":"8478_CR4","doi-asserted-by":"publisher","first-page":"1213","DOI":"10.1016\/j.fertnstert.2012.02.021","volume":"97","author":"A Cejudo Roman","year":"2012","unstructured":"Cejudo Roman, A. et al. Analysis of the expression of neurokinin B, kisspeptin, and their cognate receptors NK3R and KISS1R in the human female genital tract. Fertil. Steril. 97, 1213\u20131219 (2012).","journal-title":"Fertil. Steril."},{"key":"8478_CR5","doi-asserted-by":"crossref","unstructured":"Mei, H., Doran, J., Kyle, V., Yeo, S. H. & Colledge, W. H. Does kisspeptin signaling have a role in the testes? Front. Endocrinol. 4, 198 (2013).","DOI":"10.3389\/fendo.2013.00198"},{"key":"8478_CR6","doi-asserted-by":"crossref","unstructured":"Hu, K. L., Zhao, H., Chang, H. M., Yu, Y. & Qiao, J. Kisspeptin\/kisspeptin receptor system in the ovary. Front. Endocrinol. 8, 365 (2018).","DOI":"10.3389\/fendo.2017.00365"},{"key":"8478_CR7","doi-asserted-by":"publisher","first-page":"28969","DOI":"10.1074\/jbc.M102743200","volume":"276","author":"AI Muir","year":"2001","unstructured":"Muir, A. I. et al. AXOR12, a novel human G protein-coupled receptor, activated by the peptide KiSS-1. J. Biol. Chem. 276, 28969\u201328975 (2001).","journal-title":"J. Biol. Chem."},{"key":"8478_CR8","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/S0014-5793(99)00009-5","volume":"446","author":"DK Lee","year":"1999","unstructured":"Lee, D. K. et al. Discovery of a receptor related to the galanin receptors. FEBS Lett. 446, 103\u2013107 (1999).","journal-title":"FEBS Lett."},{"key":"8478_CR9","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1210\/en.2009-0552","volume":"151","author":"AE Herbison","year":"2010","unstructured":"Herbison, A. E., De Tassigny, X. D. A., Doran, J. & Colledge, W. H. Distribution and postnatal development of Gpr54 gene expression in mouse brain and gonadotropin-releasing hormone neurons. Endocrinology 151, 312\u2013321 (2010).","journal-title":"Endocrinology"},{"key":"8478_CR10","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1177\/0004563213485230","volume":"51","author":"CN Jayasena","year":"2014","unstructured":"Jayasena, C. N. et al. Age-dependent elevations in plasma kisspeptin are observed in boys and girls when compared with adults. Ann. Clin. Biochem.51, 89\u201396 (2014).","journal-title":"Ann. Clin. Biochem."},{"key":"8478_CR11","doi-asserted-by":"crossref","unstructured":"Lee, E. B. et al. Sexual dimorphism in Kisspeptin signaling. Cells 11, 1146 (2022).","DOI":"10.3390\/cells11071146"},{"key":"8478_CR12","doi-asserted-by":"crossref","unstructured":"Mattam, U. et al. Aging reduces kisspeptin receptor (GPR54) expression levels in the hypothalamus and extra-hypothalamic brain regions. Exp. Ther. Med. 22, 1019 (2021).","DOI":"10.3892\/etm.2021.10451"},{"key":"8478_CR13","doi-asserted-by":"crossref","unstructured":"Sliwowska, J. H. et al. Kisspeptin a potential therapeutic target in treatment of both metabolic and reproductive dysfunction. J. Diabetes 16, e13541 (2024).","DOI":"10.1111\/1753-0407.13541"},{"key":"8478_CR14","doi-asserted-by":"publisher","first-page":"4423","DOI":"10.1523\/JNEUROSCI.5352-07.2008","volume":"28","author":"C Zhang","year":"2008","unstructured":"Zhang, C., Roepke, T. A., Kelly, M. J. & R\u00f8nnekleiv, O. K. Kisspeptin depolarizes gonadotropin-releasing hormone neurons through activation of TRPC-like cationic channels. J. Neurosci. 28, 4423\u20134434 (2008).","journal-title":"J. Neurosci."},{"key":"8478_CR15","doi-asserted-by":"crossref","unstructured":"Ruohonen, S. T., Poutanen, M. & Tena-Sempere, M. Role of kisspeptins in the control of the hypothalamic-pituitary-ovarian axis: old dogmas and new challenges. Fertil. Steril. 114, 465\u2013474 (2020).","DOI":"10.1016\/j.fertnstert.2020.06.038"},{"key":"8478_CR16","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1210\/jc.2010-1359","volume":"96","author":"T Kuiri-H\u00e4nninen","year":"2011","unstructured":"Kuiri-H\u00e4nninen, T. et al. Increased activity of the hypothalamic-pituitary-testicular axis in infancy results in increased androgen action in premature boys. J. Clin. Endocrinol. Metab. 96, 98\u2013105 (2011).","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"8478_CR17","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.yhbeh.2017.06.006","volume":"94","author":"A Asaba","year":"2017","unstructured":"Asaba, A. et al. Male mice ultrasonic vocalizations enhance female sexual approach and hypothalamic kisspeptin neuron activity. Horm. Behav. 94, 53\u201360 (2017).","journal-title":"Horm. Behav."},{"key":"8478_CR18","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.neulet.2016.05.042","volume":"627","author":"R Gresham","year":"2016","unstructured":"Gresham, R. et al. Kisspeptin in the medial amygdala and sexual behavior in male rats. Neurosci. Lett. 627, 13\u201317 (2016).","journal-title":"Neurosci. Lett."},{"key":"8478_CR19","doi-asserted-by":"crossref","unstructured":"Hellier, V. et al. Female sexual behavior in mice is controlled by kisspeptin neurons. Nat. Commun. 9, 400 (2018).","DOI":"10.1038\/s41467-017-02797-2"},{"key":"8478_CR20","doi-asserted-by":"crossref","unstructured":"Kauffman, A. S. et al. The Kisspeptin receptor GPR54 is required for sexual differentiation of the brain and behavior. J. NeuroSci. 27, 8826\u201335(2007)","DOI":"10.1523\/JNEUROSCI.2099-07.2007"},{"key":"8478_CR21","doi-asserted-by":"publisher","first-page":"R1","DOI":"10.1530\/JOE-18-0269","volume":"239","author":"EGA Mills","year":"2018","unstructured":"Mills, E. G. A., Dhillo, W. S. & Comninos, A. N. Kisspeptin and the control of emotions, mood and reproductive behaviour. J. Endocrinol. 239, R1\u2013R12 (2018).","journal-title":"J. Endocrinol."},{"key":"8478_CR22","doi-asserted-by":"crossref","unstructured":"Mills, E. G. et al. Effects of Kisspeptin on sexual brain processing and penile tumescence in men with hypoactive sexual desire disorder: a randomized clinical trial. JAMA Netw. Open 6, e2254313 (2023).","DOI":"10.1001\/jamanetworkopen.2022.54313"},{"key":"8478_CR23","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1007\/978-1-4614-6199-9_3","volume":"784","author":"MN Lehman","year":"2013","unstructured":"Lehman, M. N., Hileman, S. M. & Goodman, R. L. Neuroanatomy of the kisspeptin signaling system in mammals: comparative and developmental aspects. Adv. Exp. Med Biol. 784, 27\u201362 (2013).","journal-title":"Adv. Exp. Med Biol."},{"key":"8478_CR24","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1111\/j.1365-2605.2009.01012.x","volume":"33","author":"M Tena-Sempere","year":"2010","unstructured":"Tena-Sempere, M. Kisspeptin\/GPR54 system as potential target for endocrine disruption of reproductive development and function. Int. J. Androl. 33, 360\u2013368 (2010).","journal-title":"Int. J. Androl."},{"key":"8478_CR25","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1007\/978-1-4614-6199-9_11","volume":"784","author":"SJ Semaan","year":"2013","unstructured":"Semaan, S. J., Tolson, K. P. & Kauffman, A. S. The development of kisspeptin circuits in the mammalian brain. Adv. Exp. Med. Biol. 784, 221 (2013).","journal-title":"Adv. Exp. Med. Biol."},{"key":"8478_CR26","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1507\/endocrj.EJ21-0620","volume":"69","author":"J Chen","year":"2022","unstructured":"Chen, J. et al. Kiss1-dependent and independent release of luteinizing hormone and testosterone in perinatal male rats. Endocr. J. 69, 797\u2013807 (2022).","journal-title":"Endocr. J."},{"key":"8478_CR27","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2020.612235","volume":"8","author":"L Lucaccioni","year":"2020","unstructured":"Lucaccioni, L. et al. Minipuberty: looking back to understand moving forward. Front Pediatr. 8, 612235 (2020).","journal-title":"Front Pediatr."},{"key":"8478_CR28","doi-asserted-by":"crossref","unstructured":"Alexander, G. M. Postnatal testosterone concentrations and male social development. Front. Endocrinol. 5, 15 (2014).","DOI":"10.3389\/fendo.2014.00015"},{"key":"8478_CR29","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.physbeh.2017.10.030","volume":"187","author":"MM McCarthy","year":"2018","unstructured":"McCarthy, M. M., Herold, K. & Stockman, S. L. Fast, furious and enduring: sensitive versus critical periods in sexual differentiation of the brain. Physiol. Behav. 187, 13 (2018).","journal-title":"Physiol. Behav."},{"key":"8478_CR30","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1016\/j.cell.2014.10.035","volume":"159","author":"M H\u00fcbener","year":"2014","unstructured":"H\u00fcbener, M. & Bonhoeffer, T. Neuronal plasticity: beyond the critical period. Cell 159, 727\u2013737 (2014).","journal-title":"Cell"},{"key":"8478_CR31","unstructured":"Purves, D. et al. Hormonal influences on sexual dimorphism. In: Neuroscience. 2nd edn. (2001)."},{"key":"8478_CR32","doi-asserted-by":"publisher","first-page":"86126","DOI":"10.7554\/eLife.86126","volume":"12","author":"ML Sterling","year":"2023","unstructured":"Sterling, M. L., Teunisse, R. & Englitz, B. Rodent ultrasonic vocal interaction resolved with millimeter precision using hybrid beamforming. Elife 12, 86126 (2023).","journal-title":"Elife"},{"key":"8478_CR33","doi-asserted-by":"crossref","unstructured":"Boulanger-Bertolus, J. & Mouly, A. M. Ultrasonic vocalizations emission across development in rats: coordination with respiration and impact on brain neural dynamics. Brain Sci. 11, 616 (2021).","DOI":"10.3390\/brainsci11050616"},{"key":"8478_CR34","doi-asserted-by":"crossref","unstructured":"VanRyzin, J., Marquardt, A. & McCarthy, M. Assessing rough-and-tumble play behavior in juvenile rats. Bio Protoc. 10, e3481 (2020).","DOI":"10.21769\/BioProtoc.3481"},{"key":"8478_CR35","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-21042-4","volume":"8","author":"S Delmas","year":"2018","unstructured":"Delmas, S., Porteous, R., Bergin, D. H. & Herbison, A. E. Altered aspects of anxiety-related behavior in kisspeptin receptor-deleted male mice. Sci. Rep. 8, 2794 (2018).","journal-title":"Sci. Rep."},{"key":"8478_CR36","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.regpep.2012.08.017","volume":"180","author":"M Tanaka","year":"2013","unstructured":"Tanaka, M., Csabafi, K. & Telegdy, G. Neurotransmissions of antidepressant-like effects of kisspeptin-13. Regul. Pept. 180, 1\u20134 (2013).","journal-title":"Regul. Pept."},{"key":"8478_CR37","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.bbr.2012.11.039","volume":"241","author":"K Csabafi","year":"2013","unstructured":"Csabafi, K., J\u00e1szber\u00e9nyi, M., Bagosi, Z., Lipt\u00e1k, N. & Telegdy, G. Effects of kisspeptin-13 on the hypothalamic-pituitary-adrenal axis, thermoregulation, anxiety and locomotor activity in rats. Behav. Brain Res. 241, 56\u201361 (2013).","journal-title":"Behav. Brain Res."},{"key":"8478_CR38","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1080\/10253890.2018.1438405","volume":"21","author":"O Sturman","year":"2018","unstructured":"Sturman, O., Germain, P. L. & Bohacek, J. Exploratory rearing: a context- and stress-sensitive behavior recorded in the open-field test. Stress 21, 443\u2013452 (2018).","journal-title":"Stress"},{"key":"8478_CR39","doi-asserted-by":"crossref","unstructured":"Xia, Q. et al. Elevated baseline LH\/FSH ratio is associated with poor ovulatory response but better clinical pregnancy and live birth in Chinese women with PCOS after ovulation induction. Heliyon 9, e13024 (2023).","DOI":"10.1016\/j.heliyon.2023.e13024"},{"key":"8478_CR40","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.mce.2010.01.017","volume":"324","author":"AS Kauffman","year":"2010","unstructured":"Kauffman, A. S. Coming of age in the kisspeptin era: sex differences, development, and puberty. Mol. Cell Endocrinol. 324, 51 (2010).","journal-title":"Mol. Cell Endocrinol."},{"key":"8478_CR41","doi-asserted-by":"publisher","first-page":"1774","DOI":"10.1210\/en.2006-1540","volume":"148","author":"AS Kauffman","year":"2007","unstructured":"Kauffman, A. S. et al. Sexual differentiation of Kiss1 gene expression in the brain of the rat. Endocrinology 148, 1774\u20131783 (2007).","journal-title":"Endocrinology"},{"key":"8478_CR42","doi-asserted-by":"publisher","first-page":"782","DOI":"10.1210\/en.2011-1838","volume":"153","author":"MC Poling","year":"2012","unstructured":"Poling, M. C., Kauffman, A. S., Sciences, B. & Program, G. Sexually dimorphic testosterone secretion in prenatal and neonatal mice is independent of Kisspeptin-Kiss1r and GnRH signaling. Endocrinology 153, 782\u2013793 (2012).","journal-title":"Endocrinology"},{"key":"8478_CR43","first-page":"53569","volume":"2016","author":"DN Feather-Schussler","year":"2016","unstructured":"Feather-Schussler, D. N. & Ferguson, T. S. A battery of motor tests in a neonatal mouse model of cerebral palsy. J. Vis. Exp. 2016, 53569 (2016).","journal-title":"J. Vis. Exp."},{"key":"8478_CR44","doi-asserted-by":"crossref","unstructured":"Vanryzin, J. W., Yu, S. J., Perez-Pouchoulen, M. & McCarthy, M. M. Temporary depletion of microglia during the early postnatal period induces lasting sex-dependent and sex-independent effects on behavior in rats. eNeuro 3, ENEURO.0297-16.2016 (2016).","DOI":"10.1523\/ENEURO.0297-16.2016"},{"key":"8478_CR45","doi-asserted-by":"crossref","unstructured":"R\u00f8nnekleiv, O. K., Qiu, J. & Kelly, M. J. Hypothalamic Kisspeptin neurons and the control of homeostasis. Endocrinology 163, bqab253 (2022).","DOI":"10.1210\/endocr\/bqab253"},{"key":"8478_CR46","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyg.2021.680176","volume":"12","author":"T Kikusui","year":"2021","unstructured":"Kikusui, T. et al. Testosterone increases the emission of ultrasonic vocalizations with different acoustic characteristics in mice. Front. Psychol. 12, 680176 (2021).","journal-title":"Front. Psychol."},{"key":"8478_CR47","doi-asserted-by":"publisher","first-page":"783","DOI":"10.1016\/j.physbeh.2007.11.033","volume":"93","author":"S Evans","year":"2008","unstructured":"Evans, S., Neave, N., Wakelin, D. & Hamilton, C. The relationship between testosterone and vocal frequencies in human males. Physiol. Behav. 93, 783\u2013788 (2008).","journal-title":"Physiol. Behav."},{"key":"8478_CR48","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1016\/j.neuron.2019.02.006","volume":"102","author":"JW VanRyzin","year":"2019","unstructured":"VanRyzin, J. W. et al. Microglial phagocytosis of newborn cells is induced by endocannabinoids and sculpts sex differences in juvenile rat social play. Neuron 102, 435\u2013449.e6 (2019).","journal-title":"Neuron"},{"key":"8478_CR49","doi-asserted-by":"publisher","first-page":"113450","DOI":"10.1016\/j.physbeh.2021.113450","volume":"237","author":"I Heinla","year":"2021","unstructured":"Heinla, I., Chu, X., \u00c5gmo, A. & Snoeren, E. Rat ultrasonic vocalizations and novelty-induced social and non-social investigation behavior in a seminatural environment. Physiol. Behav. 237, 113450 (2021).","journal-title":"Physiol. Behav."},{"key":"8478_CR50","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1098\/rsbl.2009.0317","volume":"5","author":"K Hammerschmidt","year":"2009","unstructured":"Hammerschmidt, K., Radyushkin, K., Ehrenreich, H. & Fischer, J. Female mice respond to male ultrasonic \u2018songs\u2019 with approach behaviour. Biol. Lett. 5, 589\u2013592 (2009).","journal-title":"Biol. Lett."},{"key":"8478_CR51","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.anbehav.2016.12.023","volume":"125","author":"JD Pultorak","year":"2017","unstructured":"Pultorak, J. D., Matusinec, K. R., Miller, Z. K. & Marler, C. A. Ultrasonic vocalization production and playback predicts intrapair and extrapair social behaviour in a monogamous mouse. Anim. Behav. 125, 13\u201323 (2017).","journal-title":"Anim. Behav."},{"key":"8478_CR52","doi-asserted-by":"publisher","first-page":"124512","DOI":"10.3389\/fnbeh.2015.00076","volume":"9","author":"J Chabout","year":"2015","unstructured":"Chabout, J., Sarkar, A., Dunson, D. B. & Jarvis, E. D. Male mice song syntax depends on socia lcontexts and influences female preferences. Front. Behav. Neurosci. 9, 124512 (2015).","journal-title":"Front. Behav. Neurosci."},{"key":"8478_CR53","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0134123","volume":"10","author":"K Musolf","year":"2015","unstructured":"Musolf, K., Meindl, S., Larsen, A. L., Kalcounis-Rueppell, M. C. & Penn, D. J. Ultrasonic vocalizations of male mice differ among species and females show assortative preferences for male calls. PLoS ONE 10, e0134123 (2015).","journal-title":"PLoS ONE"},{"key":"8478_CR54","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1016\/j.neuron.2019.05.025","volume":"103","author":"K Tschida","year":"2019","unstructured":"Tschida, K. et al. A specialized neural circuit gates social vocalizations in the mouse. Neuron 103, 459\u2013472.e4 (2019).","journal-title":"Neuron"},{"key":"8478_CR55","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2024.114528","volume":"353","author":"S Yamamoto","year":"2024","unstructured":"Yamamoto, S. et al. Kisspeptin administration may promote precopulatory behavior in male rats independently or supplementally to testosterone and contribute to proceptive behavior in female partners, reducing mating failure. Gen. Comp. Endocrinol. 353, 114528 (2024).","journal-title":"Gen. Comp. Endocrinol."},{"key":"8478_CR56","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1159\/000481137","volume":"106","author":"AN Comninos","year":"2018","unstructured":"Comninos, A. N. & Dhillo, W. S. Emerging roles of Kisspeptin in sexual and emotional brain processing. Neuroendocrinology 106, 195\u2013202 (2018).","journal-title":"Neuroendocrinology"},{"key":"8478_CR57","doi-asserted-by":"crossref","unstructured":"Comninos, A. N. et al. Kisspeptin modulates gamma-aminobutyric acid levels in the human brain. Psychoneuroendocrinology 129, 105244 (2021).","DOI":"10.1016\/j.psyneuen.2021.105244"},{"key":"8478_CR58","doi-asserted-by":"publisher","first-page":"34631","DOI":"10.1074\/jbc.M104847200","volume":"276","author":"M Kotani","year":"2001","unstructured":"Kotani, M. et al. The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan g protein-coupled receptor GPR54. J. Biol. Chem. 276, 34631\u201334636 (2001).","journal-title":"J. Biol. Chem."},{"key":"8478_CR59","doi-asserted-by":"publisher","first-page":"263655","DOI":"10.3389\/fnsys.2017.00042","volume":"11","author":"PE Micevych","year":"2017","unstructured":"Micevych, P. E. & Meisel, R. L. Integrating neural circuits controlling female sexual behavior. Front. Syst. Neurosci. 11, 263655 (2017).","journal-title":"Front. Syst. Neurosci."},{"key":"8478_CR60","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1262\/jrd.2023-019","volume":"69","author":"K Yamada","year":"2023","unstructured":"Yamada, K. et al. Sex difference in developmental changes in visualized Kiss1 neurons in newly generated Kiss1-Cre rats. J. Reprod. Dev. 69, 227\u2013238 (2023).","journal-title":"J. Reprod. Dev."},{"key":"8478_CR61","doi-asserted-by":"publisher","DOI":"10.1111\/jne.12590","volume":"30","author":"A Acevedo-Rodriguez","year":"2018","unstructured":"Acevedo-Rodriguez, A. et al. Emerging insights into hypothalamic-pituitary-gonadal axis regulation and interaction with stress signalling. J. Neuroendocrinol. 30, e12590 (2018).","journal-title":"J. Neuroendocrinol."},{"key":"8478_CR62","doi-asserted-by":"crossref","unstructured":"Santi, D. et al. Follicle-stimulating hormone (FSH) action on spermatogenesis: a focus on physiological and therapeutic roles. J.Clin. Med. 9, 1014 (2020).","DOI":"10.3390\/jcm9041014"},{"key":"8478_CR63","doi-asserted-by":"publisher","first-page":"13805","DOI":"10.1073\/pnas.1509591112","volume":"112","author":"SM Van Anders","year":"2015","unstructured":"Van Anders, S. M., Steiger, J. & Goldey, K. L. Effects of gendered behavior on testosterone in women and men. Proc. Natl. Acad. Sci. USA 112, 13805\u201313810 (2015).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"8478_CR64","doi-asserted-by":"crossref","unstructured":"Torres, E. et al. Kisspeptin signaling in astrocytes modulates the reproductive axis. J. Clin. Invest. 134, e172908 (2024).","DOI":"10.1172\/JCI172908"},{"key":"8478_CR65","doi-asserted-by":"publisher","first-page":"2380","DOI":"10.1210\/jc.2015-4175","volume":"101","author":"M Taziaux","year":"2016","unstructured":"Taziaux, M. et al. KisspEptin expression in the human infundibular nucleus in relation to sex, gender identity, and sexual orientation. J. Clin. Endocrinol. Metab. 101, 2380\u20132389 (2016).","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"8478_CR66","doi-asserted-by":"publisher","first-page":"3920","DOI":"10.1523\/JNEUROSCI.5740-08.2009","volume":"29","author":"AK Roseweir","year":"2009","unstructured":"Roseweir, A. K. et al. Discovery of potent kisspeptin antagonists delineate physiological mechanisms of gonadotropin regulation. J. Neurosci. 29, 3920\u20133929 (2009).","journal-title":"J. Neurosci."},{"key":"8478_CR67","doi-asserted-by":"publisher","first-page":"1325","DOI":"10.1111\/2041-210X.12584","volume":"7","author":"O Friard","year":"2016","unstructured":"Friard, O. & Gamba, M. BORIS: a free, versatile open-source event-logging software for video\/audio coding and live observations. Methods Ecol. Evol. 7, 1325\u20131330 (2016).","journal-title":"Methods Ecol. Evol."}],"container-title":["Communications Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s42003-025-08478-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s42003-025-08478-x","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s42003-025-08478-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,7]],"date-time":"2025-09-07T21:06:07Z","timestamp":1757279167000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s42003-025-08478-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,22]]},"references-count":67,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["8478"],"URL":"https:\/\/doi.org\/10.1038\/s42003-025-08478-x","relation":{},"ISSN":["2399-3642"],"issn-type":[{"value":"2399-3642","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,22]]},"assertion":[{"value":"27 September 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 July 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"1085"}}