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Herein, inspired by the biological cooperativity of proteins in macro-molecularly crowded environments, these restrictions have been overcome. Using ionic liquid cholinium tosylate that acts as a fibrillation agent, instantaneous production of protein fibrils is shown directly from a real and low-cost matrix, i.e. egg white. The fibrillation of egg white proteome is confirmed by microscopy, whereas the fibrillation kinetics is monitored by fluorescence changes of the thioflavin T dye and secondary structural transitions. Spectroscopic and molecular docking studies are used to identify the proteins involved and to appraise the molecular-level mechanisms ruling the proteins structural changes upon fibrillation. The obtained fibrils have enhanced mechanical stiffness and cytocompatibility, demonstrating their potential to act as improved enzyme supports.<\/jats:p>","DOI":"10.1038\/s43246-020-0035-0","type":"journal-article","created":{"date-parts":[[2020,6,11]],"date-time":"2020-06-11T10:02:41Z","timestamp":1591869761000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Instantaneous fibrillation of egg white proteome with ionic liquid and macromolecular crowding"],"prefix":"10.1038","volume":"1","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6573-0475","authenticated-orcid":false,"given":"Pankaj","family":"Bharmoria","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1715-5514","authenticated-orcid":false,"given":"Dibyendu","family":"Mondal","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3524-6900","authenticated-orcid":false,"given":"Matheus M.","family":"Pereira","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5611-4080","authenticated-orcid":false,"given":"M\u00e1rcia C.","family":"Neves","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2101-8309","authenticated-orcid":false,"given":"Mafalda R.","family":"Almeida","sequence":"additional","affiliation":[]},{"given":"Maria C.","family":"Gomes","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o F.","family":"Mano","sequence":"additional","affiliation":[]},{"given":"Igor","family":"Bdikin","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1085-7836","authenticated-orcid":false,"given":"Rute A. 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