{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T06:45:04Z","timestamp":1775198704520,"version":"3.50.1"},"reference-count":72,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T00:00:00Z","timestamp":1706054400000},"content-version":"vor","delay-in-days":158,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T00:00:00Z","timestamp":1692403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T00:00:00Z","timestamp":1692403200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Research on marine microbial communities is growing, but studies are hard to compare because of variation in seawater sampling protocols. To help researchers in the inter-comparison of studies that use different seawater sampling methodologies, as well as to help them design future sampling campaigns, we developed the EuroMarine Open Science Exploration initiative (EMOSE). Within the EMOSE framework, we sampled thousands of liters of seawater from a single station in the NW Mediterranean Sea (Service d\u2019Observation du Laboratoire Arago [SOLA], Banyuls-sur-Mer), during one single day. The resulting dataset includes multiple seawater processing approaches, encompassing different material-type kinds of filters (cartridge membrane and flat membrane), three different size fractionations (&amp;gt;0.22\u2009\u00b5m, 0.22\u20133\u2009\u00b5m, 3\u201320\u2009\u00b5m and &amp;gt;20\u2009\u00b5m), and a number of different seawater volumes ranging from 1\u2009L up to 1000\u2009L. We show that the volume of seawater that is filtered does not have a significant effect on prokaryotic and protist diversity, independently of the sequencing strategy. However, there was a clear difference in alpha and beta diversity between size fractions and between these and \u201cwhole water\u201d (with no pre-fractionation). Overall, we recommend care when merging data from datasets that use filters of different pore size, but we consider that the type of filter and volume should not act as confounding variables for the tested sequencing strategies. To the best of our knowledge, this is the first time a publicly available dataset effectively allows for the clarification of the impact of marine microbiome methodological options across a wide range of protocols, including large-scale variations in sampled volume.<\/jats:p>","DOI":"10.1038\/s43705-023-00278-w","type":"journal-article","created":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T08:03:22Z","timestamp":1692432202000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Inter-comparison of marine microbiome sampling protocols"],"prefix":"10.1093","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2315-358X","authenticated-orcid":false,"given":"Francisco","family":"Pascoal","sequence":"first","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research, University of Porto , Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. 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Passeig Mar\u00edtim de la Barceloneta , 37-49, ES08003 Barcelona, Spain"}]},{"given":"Francesca","family":"Malfatti","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Trieste , Trieste, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0016-1624","authenticated-orcid":false,"given":"Hisashi","family":"Endo","sequence":"additional","affiliation":[{"name":"Bioinformatics Center, Institute for Chemical Research, Kyoto University , Gokasho, Uji, Japan"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0911-3082","authenticated-orcid":false,"given":"Anna Maria","family":"D\u0105browska","sequence":"additional","affiliation":[{"name":"Department of Marine Ecology, Institute of Oceanology Polish Academy of Sciences , Sopot, Poland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8390-1300","authenticated-orcid":false,"given":"Fabio","family":"De Pascale","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Padua , Via U. 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