{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T21:53:08Z","timestamp":1768773188147,"version":"3.49.0"},"reference-count":67,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T00:00:00Z","timestamp":1704844800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T00:00:00Z","timestamp":1704844800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100019180","name":"EC | ERC | HORIZON EUROPE European Research Council","doi-asserted-by":"publisher","award":["ERC-2015-CoG-683258"],"award-info":[{"award-number":["ERC-2015-CoG-683258"]}],"id":[{"id":"10.13039\/100019180","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"MEC | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BIA-BID\/32225\/2017"],"award-info":[{"award-number":["PTDC\/BIA-BID\/32225\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"MEC | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BIA-BID\/32225\/2017"],"award-info":[{"award-number":["PTDC\/BIA-BID\/32225\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["EMBO Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Centrioles are part of centrosomes and cilia, which are microtubule organising centres (MTOC) with diverse functions. Despite their stability, centrioles can disappear during differentiation, such as in oocytes, but little is known about the regulation of their structural integrity. Our previous research revealed that the pericentriolar material (PCM) that surrounds centrioles and its recruiter, Polo kinase, are downregulated in oogenesis and sufficient for maintaining both centrosome structural integrity and MTOC activity. We now show that the expression of specific components of the centriole cartwheel and wall, including ANA1\/CEP295, is essential for maintaining centrosome integrity. We find that Polo kinase requires ANA1 to promote centriole stability in cultured cells and eggs. In addition, ANA1 expression prevents the loss of centrioles observed upon PCM-downregulation. However, the centrioles maintained by overexpressing and tethering ANA1 are inactive, unlike the MTOCs observed upon tethering Polo kinase. These findings demonstrate that several centriole components are needed to maintain centrosome structure. Our study also highlights that centrioles are more dynamic than previously believed, with their structural stability relying on the continuous expression of multiple components.<\/jats:p>","DOI":"10.1038\/s44319-023-00020-6","type":"journal-article","created":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T08:18:14Z","timestamp":1705047494000},"page":"102-127","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Ana1\/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM"],"prefix":"10.1038","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6318-3922","authenticated-orcid":false,"given":"Ana","family":"Pimenta-Marques","sequence":"first","affiliation":[]},{"given":"Tania","family":"Perestrelo","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1681-508X","authenticated-orcid":false,"given":"Patricia","family":"Reis-Rodrigues","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1833-5629","authenticated-orcid":false,"given":"Paulo","family":"Duarte","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2938-141X","authenticated-orcid":false,"given":"Ana","family":"Ferreira-Silva","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9842-173X","authenticated-orcid":false,"given":"Mariana","family":"Lince-Faria","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1987-5598","authenticated-orcid":false,"given":"M\u00f3nica","family":"Bettencourt-Dias","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2024,1,10]]},"reference":[{"issue":"14","key":"20_CR1","doi-asserted-by":"publisher","first-page":"jcs258987","DOI":"10.1242\/jcs.258987","volume":"134","author":"I Alvarez-Rodrigo","year":"2021","unstructured":"Alvarez-Rodrigo I, Wainman A, Saurya S, Raff JW (2021) Ana1 helps recruit Polo to centrioles to promote mitotic PCM assembly and centriole elongation. J Cell Sci 134(14):jcs258987","journal-title":"J Cell Sci"},{"issue":"18","key":"20_CR2","doi-asserted-by":"publisher","first-page":"3125","DOI":"10.1242\/jcs.064782","volume":"123","author":"S Bahmanyar","year":"2010","unstructured":"Bahmanyar S, Guiney EL, Hatch EM, Nelson WJ, Barth AIM (2010) Formation of extra centrosomal structures is dependent on \u03b2-catenin. J Cell Sci 123(18):3125\u20133135","journal-title":"J Cell Sci"},{"key":"20_CR3","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1038\/cr.2015.49","volume":"25","author":"FR Balestra","year":"2015","unstructured":"Balestra FR, Von Tobel L, G\u00f6nczy P (2015) Paternally contributed centrioles exhibit exceptional persistence in C. elegans embryos. Cell Res 25:642\u2013644","journal-title":"Cell Res"},{"issue":"3","key":"20_CR4","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1016\/0092-8674(90)90022-7","volume":"62","author":"LD Belmont","year":"1990","unstructured":"Belmont LD, Hyman AA, Sawin KE, Mitchison TJ (1990) Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts. Cell 62(3):579\u2013589","journal-title":"Cell"},{"issue":"7020","key":"20_CR5","doi-asserted-by":"publisher","first-page":"980","DOI":"10.1038\/nature03160","volume":"432","author":"M Bettencourt-Dias","year":"2004","unstructured":"Bettencourt-Dias M, Giet R, Sinka R, Mazumdar A, Lock WG, Balloux F, Zafiropoulos PJ, Yamaguchi S, Winter S, Carthew RW et al (2004) Genome-wide survey of protein kinases required for cell cycle progression. Nature 432(7020):980\u2013987","journal-title":"Nature"},{"key":"20_CR6","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1007\/978-1-60327-993-2_3","volume":"545","author":"M Bettencourt-Dias","year":"2009","unstructured":"Bettencourt-Dias M, Goshima G (2009) RNAi in drosophila S2 cells as a tool for studying cell cycle progression. Methods Mol Biol 545:39\u201362.","journal-title":"Methods Mol Biol"},{"issue":"24","key":"20_CR7","doi-asserted-by":"publisher","first-page":"2199","DOI":"10.1016\/j.cub.2005.11.042","volume":"15","author":"M Bettencourt-Dias","year":"2005","unstructured":"Bettencourt-Dias M, Rodrigues-Martins A, Carpenter L, Riparbelli M, Lehmann L, Gatt MK, Carmo N, Balloux F, Callaini G, Glover DM (2005) SAK\/PLK4 is required for centriole duplication and flagella development. Curr Biol 15(24):2199\u20132207","journal-title":"Curr Biol"},{"issue":"1","key":"20_CR8","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1534\/genetics.109.101709","volume":"182","author":"S Blachon","year":"2009","unstructured":"Blachon S, Cai X, Roberts KA, Yang K, Polyanovsky A, Church A, Avidor-Reiss T (2009) A proximal centriole-like structure is present in drosophila spermatids and can serve as a model to study centriole duplication. Genetics 182(1):133\u2013144","journal-title":"Genetics"},{"issue":"4","key":"20_CR9","doi-asserted-by":"publisher","first-page":"2081","DOI":"10.1534\/genetics.108.095141","volume":"180","author":"S Blachon","year":"2008","unstructured":"Blachon S, Gopalakrishnan J, Omori Y, Polyanovsky A, Church A, Nicastro D, Malicki J, Avidor-Reiss T (2008) Drosophila asterless and vertebrate Cep152 are orthologs essential for centriole duplication. Genetics 180(4):2081\u20132094","journal-title":"Genetics"},{"key":"20_CR10","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1016\/j.ceb.2012.01.003","volume":"24","author":"DA Brito","year":"2012","unstructured":"Brito DA, Gouveia SM, Bettencourt-Dias M (2012) Deconstructing the centriole: structure and number control. Curr Opin Cell Biol 24:4\u201313","journal-title":"Curr Opin Cell Biol"},{"key":"20_CR11","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1016\/j.devcel.2019.06.004","volume":"50","author":"G Cabral","year":"2019","unstructured":"Cabral G, Laos T, Dumont J, Dammermann A (2019) Differential requirements for centrioles in mitotic centrosome growth and maintenance. Dev Cell 50:355\u2013366.e6","journal-title":"Dev Cell"},{"issue":"1","key":"20_CR12","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1006\/excr.1997.3618","volume":"234","author":"G Callaini","year":"1997","unstructured":"Callaini G, Whitfield WGF, Riparbelli MG (1997) Centriole and centrosome dynamics during the embryonic cell cycles that follow the formation of the cellular blastoderm in Drosophila. Exp Cell Res 234(1):183\u2013190","journal-title":"Exp Cell Res"},{"issue":"2","key":"20_CR13","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1016\/j.devcel.2012.06.001","volume":"23","author":"Z Carvalho-Santos","year":"2012","unstructured":"Carvalho-Santos Z, Machado P, Alvarez-Martins I, Gouveia SM, Jana SC, Duarte P, Amado T, Branco P, Freitas MC, Silva STN et al (2012) BLD10\/CEP135 is a microtubule-associated protein that controls the formation of the Flagellum central microtubule pair. Dev Cell 23(2):412\u2013424","journal-title":"Dev Cell"},{"issue":"1","key":"20_CR14","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1038\/nsmb.2180","volume":"19","author":"E Caussinus","year":"2012","unstructured":"Caussinus E, Kanca O, Affolter M (2012) Fluorescent fusion protein knockout mediated by anti-GFP nanobody. Nat Struct Mol Biol 19(1):117\u2013121","journal-title":"Nat Struct Mol Biol"},{"issue":"13","key":"20_CR15","doi-asserted-by":"crossref","first-page":"2501","DOI":"10.1242\/jcs.186338","volume":"129","author":"CW Chang","year":"2016","unstructured":"Chang CW, Hsu WB, Tsai JJ, Tang CJC, Tang TK (2016) CEP295 interacts with microtubules and is required for centriole elongation. J Cell Sci 129(13):2501\u20132513","journal-title":"J Cell Sci"},{"key":"20_CR16","doi-asserted-by":"publisher","first-page":"e08483","DOI":"10.7554\/eLife.08483","volume":"4","author":"PT Conduit","year":"2015","unstructured":"Conduit PT, Wainman A, Novak ZA, Weil TT, Raff JW (2015) Re-examining the role of Drosophila Sas-4 in centrosome assembly using two-colour-3D-SIM FRAP. ELife 4:e08483","journal-title":"ELife"},{"key":"20_CR17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.7554\/eLife.07236","volume":"4","author":"MA Cottee","year":"2015","unstructured":"Cottee MA, Muschalik N, Johnson S, Leveson J, Raff JW, Lea SM (2015) The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies. ELife 4:1\u201365","journal-title":"ELife"},{"issue":"9","key":"20_CR18","doi-asserted-by":"publisher","first-page":"e224","DOI":"10.1371\/journal.pbio.0060224","volume":"6","author":"J Dobbelaere","year":"2008","unstructured":"Dobbelaere J, Josu\u00e9 F, Suijkerbuijk S, Baum B, Tapon N, Raff J (2008) A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila. PLoS Biol 6(9):e224\u2013e224","journal-title":"PLoS Biol"},{"issue":"21","key":"20_CR19","doi-asserted-by":"publisher","first-page":"2526","DOI":"10.1016\/j.cub.2014.08.061","volume":"24","author":"NS Dzhindzhev","year":"2014","unstructured":"Dzhindzhev NS, Tzolovsky G, Lipinszki Z, Schneider S, Lattao R, Fu J, Debski J, Debski J, Dadlez M, Glover DM (2014) Plk4 phosphorylates ana2 to trigger SAS6 recruitment and procentriole formation. Curr Biol 24(21):2526\u20132532","journal-title":"Curr Biol"},{"issue":"7316","key":"20_CR20","doi-asserted-by":"publisher","first-page":"714","DOI":"10.1038\/nature09445","volume":"467","author":"NS Dzhindzhev","year":"2010","unstructured":"Dzhindzhev NS, Yu QD, Weiskopf K, Tzolovsky G, Cunha-Ferreira I, Riparbelli M, Rodrigues-Martins A, Bettencourt-Dias M, Callaini G, Glover DM (2010a) Asterless is a scaffold for the onset of centriole assembly. Nature 467(7316):714\u2013718","journal-title":"Nature"},{"issue":"8","key":"20_CR21","doi-asserted-by":"publisher","first-page":"120104","DOI":"10.1098\/rsob.120104","volume":"2","author":"J Fu","year":"2012","unstructured":"Fu J, Glover DM (2012) Structured illumination of the interface between centriole and peri-centriolar material. Open Biol 2(8):120104","journal-title":"Open Biol"},{"issue":"1","key":"20_CR22","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1038\/ncb3274","volume":"18","author":"J Fu","year":"2016","unstructured":"Fu J, Lipinszki Z, Rangone H, Min M, Mykura C, Chao-Chu J, Schneider S, Dzhindzhev NS, Gottardo M, Riparbelli MG et al (2016) Conserved molecular inteactions in centriole-to-centrosome conversion. Nat Cell Biol 18(1):87\u201399","journal-title":"Nat Cell Biol"},{"issue":"1","key":"20_CR23","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12476","volume":"7","author":"BJ Galletta","year":"2016","unstructured":"Galletta BJ, Fagerstrom CJ, Schoborg TA, McLamarrah TA, Ryniawec JM, Buster DW, Slep KC, Rogers GC, Rusan NM (2016) A centrosome interactome provides insight into organelle assembly and reveals a non-duplication role for Plk4. Nat Commun 7(1):12476","journal-title":"Nat Commun"},{"issue":"6","key":"20_CR24","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1093\/embo-reports\/kvd105","volume":"1","author":"AK Gillingham","year":"2000","unstructured":"Gillingham AK, Munro S (2000) The PACT domain, a conserved centrosomal targeting motif in the coiled\u2010coil proteins AKAP450 and pericentrin. EMBO Rep 1(6):524\u2013529","journal-title":"EMBO Rep"},{"issue":"1","key":"20_CR25","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1367","volume":"2","author":"J Gopalakrishnan","year":"2011","unstructured":"Gopalakrishnan J, Mennella V, Blachon S, Zhai B, Smith AH, Megraw TL, Nicastro D, Gygi SP, Agard DA, Avidor-Reiss T (2011) Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome. Nat Commun 2(1):359","journal-title":"Nat Commun"},{"issue":"4","key":"20_CR26","doi-asserted-by":"publisher","first-page":"1700241","DOI":"10.1002\/bies.201700241","volume":"40","author":"P Guichard","year":"2018","unstructured":"Guichard P, Hamel V, G\u00f6nczy P (2018) The Rise of the Cartwheel: Seeding the Centriole Organelle. BioEssays 40(4):1700241","journal-title":"BioEssays"},{"issue":"11","key":"20_CR27","doi-asserted-by":"publisher","first-page":"1140","DOI":"10.1038\/ncb1320","volume":"7","author":"R Habedanck","year":"2005","unstructured":"Habedanck R, Stierhof Y-D, Wilkinson CJ, Nigg EA (2005) The Polo kinase Plk4 functions in centriole duplication. Nat Cell Biol 7(11):1140\u20131146","journal-title":"Nat Cell Biol"},{"issue":"6","key":"20_CR28","doi-asserted-by":"publisher","first-page":"e5976","DOI":"10.1371\/journal.pone.0005976","volume":"4","author":"L Haren","year":"2009","unstructured":"Haren L, Stearns T, L\u00fcders J (2009) Plk1-Dependent recruitment of \u03b3-tubulin complexes to mitotic centrosomes involves multiple PCM components. PLoS ONE 4(6):e5976","journal-title":"PLoS ONE"},{"issue":"4","key":"20_CR29","doi-asserted-by":"publisher","first-page":"957","DOI":"10.1016\/j.celrep.2014.07.022","volume":"8","author":"D Izquierdo","year":"2014","unstructured":"Izquierdo D, Wang W-J, Uryu K, Tsou M-FB (2014) Stabilization of cartwheel-less centrioles for duplication requires CEP295-mediated centriole-to-centrosome conversion. Cell Rep 8(4):957\u2013965","journal-title":"Cell Rep"},{"issue":"4","key":"20_CR30","doi-asserted-by":"publisher","first-page":"e05003","DOI":"10.7554\/eLife.05003","volume":"2015","author":"H Jambor","year":"2015","unstructured":"Jambor H, Surendranath V, Kalinka AT, Mejstrik P, Saalfeld S, Tomancak P (2015) \u00a7Systematic imaging reveals features and changing localization of mRNAs in Drosophila development. ELife 2015(4):e05003","journal-title":"ELife"},{"issue":"5","key":"20_CR31","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1083\/jcb.201307049","volume":"204","author":"D Keller","year":"2014","unstructured":"Keller D, Orpinell M, Olivier N, Wachsmuth M, Mahen R, Wyss R, Hachet V, Ellenberg J, Manley S, Gonczy P (2014) Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells. J Cell Biol 204(5):697\u2013712","journal-title":"J Cell Biol"},{"issue":"23","key":"20_CR32","doi-asserted-by":"publisher","first-page":"3183","DOI":"10.1016\/j.cub.2016.07.006","volume":"26","author":"A Khire","year":"2016","unstructured":"Khire A, Jo KH, Kong D, Akhshi T, Blachon S, Cekic AR, Hynek S, Ha A, Loncarek J, Mennella V et al (2016) Centriole remodeling during spermiogenesis in Drosophila. Curr Biol 26(23):3183\u20133189","journal-title":"Curr Biol"},{"issue":"3","key":"20_CR33","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1016\/j.cell.2011.01.008","volume":"144","author":"D Kitagawa","year":"2011","unstructured":"Kitagawa D, Vakonakis I, Olieric N, Hilbert M, Keller D, Olieric V, Bortfeld M, Erat MC, Fluckiger I, Gonczy P et al (2011) Structural basis of the 9-fold symmetry of centrioles. Cell 144(3):364\u2013375","journal-title":"Cell"},{"issue":"2","key":"20_CR34","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1016\/j.devcel.2007.07.002","volume":"13","author":"J Kleylein-Sohn","year":"2007","unstructured":"Kleylein-Sohn J, Westendorf J, Le Clech M, Habedanck R, Stierhof YD, Nigg EA (2007) Plk4-induced centriole biogenesis in human cells. Dev Cell 13(2):190\u2013202","journal-title":"Dev Cell"},{"issue":"5","key":"20_CR35","doi-asserted-by":"publisher","first-page":"1599","DOI":"10.1083\/jcb.110.5.1599","volume":"110","author":"RS Kochanski","year":"1990","unstructured":"Kochanski RS, Borisy GG (1990) Mode of centriole duplication and distribution. J Cell Biol 110(5):1599\u20131605","journal-title":"J Cell Biol"},{"issue":"6","key":"20_CR36","doi-asserted-by":"publisher","first-page":"1701","DOI":"10.1083\/jcb.135.6.1701","volume":"135","author":"HA Lane","year":"1996","unstructured":"Lane HA, Nigg EA (1996) Antibody microinjection reveals an essential role for human polo-like kinase 1 (Plk1) in the functional maturation of mitotic centrosomes. J Cell Biol 135(6):1701\u20131713","journal-title":"J Cell Biol"},{"issue":"20","key":"20_CR37","doi-asserted-by":"publisher","first-page":"3994","DOI":"10.1242\/dev.111054","volume":"141","author":"N Lowe","year":"2014","unstructured":"Lowe N, Rees JS, Roote J, Ryder E, Armean IM, Johnson G, Drummond E, Magbanua JP, Naylor H, Sanson B et al (2014) Analysis of the expression patterns, subcellular localisations and interaction partners of drosophila proteins using a pigp protein trap library. Development 141(20):3994\u20134005","journal-title":"Development"},{"issue":"2","key":"20_CR38","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1083\/jcb.201003009","volume":"191","author":"MR Mahjoub","year":"2010","unstructured":"Mahjoub MR, Xie Z, Stearns T (2010) Cep120 is asymmetrically localized to the daughter centriole and is essential for centriole assembly. J Cell Biol 191(2):331\u2013346","journal-title":"J Cell Biol"},{"issue":"2","key":"20_CR39","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1083\/jcb.45.2.306","volume":"45","author":"AP Mahowald","year":"1970","unstructured":"Mahowald AP, Strassheim JM (1970) Intercellular migration of centrioles in the germarium of Drosophila melanogaster. An electron microscopic study. J Cell Biol 45(2):306\u2013320","journal-title":"J Cell Biol"},{"issue":"1","key":"20_CR40","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1095\/biolreprod.104.031245","volume":"72","author":"G Manandhar","year":"2005","unstructured":"Manandhar G, Schatten H, Sutovsky P (2005) Centrosome reduction during gametogenesis and its significance. Biol Reprodn 72(1):2\u201313","journal-title":"Biol Reprodn"},{"issue":"5","key":"20_CR41","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1083\/jcb.200402130","volume":"165","author":"M Martinez-Campos","year":"2004","unstructured":"Martinez-Campos M, Basto R, Baker J, Kernan M, Raff JW (2004) The Drosophila pericentrin-like protein is essential for cilia\/flagella function, but appears to be dispensable for mitosis. J Cell Biol 165(5):673\u2013683","journal-title":"J Cell Biol"},{"key":"20_CR42","doi-asserted-by":"crossref","unstructured":"Mazo G. (2021) QuickFigures: A toolkit and ImageJ PlugIn to quickly transform microscope images into scientific figures. PLoS One 16(11):e0240280.","DOI":"10.1371\/journal.pone.0240280"},{"issue":"3","key":"20_CR43","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1016\/j.tcb.2013.10.001","volume":"24","author":"V Mennella","year":"2014","unstructured":"Mennella V, Agard DA, Huang B, Pelletier L (2014) Amorphous no more: subdiffraction view of the pericentriolar material architecture. Trends Cell Biol 24(3):188\u2013197","journal-title":"Trends Cell Biol"},{"issue":"11","key":"20_CR44","doi-asserted-by":"publisher","first-page":"1159","DOI":"10.1038\/ncb2597","volume":"14","author":"V Mennella","year":"2012","unstructured":"Mennella V, Keszthelyi B, McDonald KL, Chhun B, Kan F, Rogers GC, Huang B, Agard DA (2012) Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization. Nat Cell Biol 14(11):1159\u20131168","journal-title":"Nat Cell Biol"},{"key":"20_CR45","doi-asserted-by":"crossref","unstructured":"Mottier-Pavie V, Megraw TL. (2009) Drosophila Bld10 is a centriolar protein that regulates centriole, basal body, and motile cilium assembly. Mol Biol Cell 20(10):2605\u20132614","DOI":"10.1091\/mbc.e08-11-1115"},{"key":"20_CR46","doi-asserted-by":"publisher","first-page":"e46054","DOI":"10.7554\/eLife.46054","volume":"8","author":"TC Moyer","year":"2019","unstructured":"Moyer TC, Holland AJ (2019) Plk4 promotes centriole duplication by phosphorylating stil to link the procentriole cartwheel to the microtubule wall. ELife 8:e46054","journal-title":"ELife"},{"issue":"17","key":"20_CR47","doi-asserted-by":"publisher","first-page":"3012","DOI":"10.1242\/dev.153171","volume":"144","author":"A Muroyama","year":"2017","unstructured":"Muroyama A, Lechler T (2017) Microtubule organization, dynamics and functions in differentiated cells. Development 144(17):3012\u20133021","journal-title":"Development"},{"issue":"5","key":"20_CR48","doi-asserted-by":"publisher","first-page":"e202008090","DOI":"10.1083\/jcb.202008090","volume":"220","author":"C Nabais","year":"2021","unstructured":"Nabais C, Pessoa D, de-Carvalho J, van Zanten T, Duarte P, Mayor S, Carneiro J, Telley IA, Bettencourt-Dias M (2021) Plk4 triggers autonomous de novo centriole biogenesis and maturation. J Cell Biol 220(5):e202008090","journal-title":"J Cell Biol"},{"issue":"11","key":"20_CR49","doi-asserted-by":"publisher","first-page":"1276","DOI":"10.1016\/j.cub.2014.04.023","volume":"24","author":"ZA Novak","year":"2014","unstructured":"Novak ZA, Conduit PT, Wainman A, Raff JW (2014) Asterless licenses daughter centrioles to duplicate for the first time in Drosophila embryos. Curr Biol 24(11):1276\u20131282","journal-title":"Curr Biol"},{"issue":"6294","key":"20_CR50","doi-asserted-by":"publisher","first-page":"aaf4866","DOI":"10.1126\/science.aaf4866","volume":"353","author":"A Pimenta-Marques","year":"2016","unstructured":"Pimenta-Marques A, Bento I, Lopes CAM, Duarte P, Jana SC, Bettencourt-Dias M (2016) A mechanism for the elimination of the female gamete centrosome in Drosophila melanogaster. Science 353(6294):aaf4866","journal-title":"Science"},{"key":"20_CR51","doi-asserted-by":"crossref","unstructured":"Pimenta-Marques A, Bettencourt-Dias M (2020) Pericentriolar material. Current Biology 30(12):R687-R689","DOI":"10.1016\/j.cub.2020.04.064"},{"issue":"4","key":"20_CR52","doi-asserted-by":"publisher","first-page":"jcs211441","DOI":"10.1242\/jcs.211441","volume":"131","author":"MG Riparbelli","year":"2018","unstructured":"Riparbelli MG, Persico V, Gottardo M, Callaini G (2018) The developing Drosophila eye - A new model to study centriole reduction. J Cell Sci 131(4):jcs211441","journal-title":"J Cell Sci"},{"issue":"23","key":"20_CR53","doi-asserted-by":"publisher","first-page":"5881","DOI":"10.1242\/jcs.113506","volume":"125","author":"H Roque","year":"2012","unstructured":"Roque H, Wainman A, Richens J, Kozyrska K, Franz A, Raff JW (2012) Drosophila Cep135\/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation. J Cell Sci 125(23):5881\u20135886","journal-title":"J Cell Sci"},{"issue":"7","key":"20_CR54","doi-asserted-by":"publisher","first-page":"879","DOI":"10.1016\/j.cub.2015.01.066","volume":"25","author":"D Sabino","year":"2015","unstructured":"Sabino D, Gogendeau D, Gambarotto D, Nano M, Pennetier C, Dingli F, Arras G, Loew D, Basto R (2015) Moesin is a major regulator of centrosome behavior in epithelial cells with extra centrosomes. Curr Biol 25(7):879\u2013889","journal-title":"Curr Biol"},{"issue":"3","key":"20_CR55","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1016\/j.jmb.2005.07.038","volume":"352","author":"D Saerens","year":"2005","unstructured":"Saerens D, Pellis M, Loris R, Pardon E, Dumoulin M, Matagne A, Wyns L, Muyldermans S, Conrath K (2005) Identification of a universal VHH framework to graft non-canonical antigen-binding loops of camel single-domain antibodies. J Mol Biol 352(3):597\u2013607","journal-title":"J Mol Biol"},{"key":"20_CR56","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.ceb.2016.09.003","volume":"44","author":"AD Sanchez","year":"2017","unstructured":"Sanchez AD, Feldman JL (2017) Microtubule-organizing centers: from the centrosome to non-centrosomal sites. Curr Opin Cell Biol 44:93\u2013101","journal-title":"Curr Opin Cell Biol"},{"issue":"13","key":"20_CR57","doi-asserted-by":"crossref","first-page":"2514 LP","DOI":"10.1242\/jcs.186460","volume":"129","author":"S Saurya","year":"2016","unstructured":"Saurya S, Roque H, Novak ZA, Wainman A, Aydogan MG, Volanakis A, Sieber B, Pinto DMS, Raff JW (2016) Drosophila Ana1 is required for centrosome assembly and centriole elongation. J Cell Sci 129(13):2514 LP\u20132512525","journal-title":"J Cell Sci"},{"issue":"13","key":"20_CR58","doi-asserted-by":"publisher","first-page":"1548","DOI":"10.1016\/j.cub.2014.05.050","volume":"24","author":"P Singh","year":"2014","unstructured":"Singh P, Ramdas Nair A, Cabernard C (2014) The centriolar protein Bld10\/Cep135 is required to establish centrosome asymmetry in drosophila neuroblasts. Curr Biol 24(13):1548\u20131555","journal-title":"Curr Biol"},{"issue":"4","key":"20_CR59","doi-asserted-by":"publisher","first-page":"e202005103","DOI":"10.1083\/jcb.202005103","volume":"220","author":"Y Tian","year":"2021","unstructured":"Tian Y, Wei C, He J, Yan Y, Pang N, Fang X, Liang X, Fu J (2021) Superresolution characterization of core centriole architecture. J Cell Biol 220(4):e202005103","journal-title":"J Cell Biol"},{"issue":"9","key":"20_CR60","doi-asserted-by":"publisher","first-page":"121","DOI":"10.3390\/cells7090121","volume":"7","author":"M Tillery","year":"2018","unstructured":"Tillery M, Blake-Hedges C, Zheng Y, Buchwalter R, Megraw T (2018) Centrosomal and non-centrosomal microtubule-organizing centers (MTOCs) in Drosophila melanogaster. Cells 7(9):121","journal-title":"Cells"},{"key":"20_CR61","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12567","volume":"7","author":"Y Tsuchiya","year":"2016","unstructured":"Tsuchiya Y, Yoshiba S, Gupta A, Watanabe K, Kitagawa D (2016) Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole. Nat Commun 7:12567","journal-title":"Nat Commun"},{"issue":"22","key":"20_CR62","doi-asserted-by":"publisher","first-page":"3789","DOI":"10.1242\/jcs.203505","volume":"130","author":"S Werner","year":"2017","unstructured":"Werner S, Pimenta-Marques A, Bettencourt-Dias M (2017) Maintaining centrosomes and cilia. J Cell Sci 130(22):3789\u20133800","journal-title":"J Cell Sci"},{"issue":"6239","key":"20_CR63","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1126\/science.aaa5111","volume":"348","author":"YL Wong","year":"2015","unstructured":"Wong YL, Anzola JV, Davis RL, Yoon M, Motamedi A, Kroll A, Seo CP, Hsia JE, Kim SK, Mitchell JW et al (2015) Reversible centriole depletion with an inhibitor of Polo-like kinase 4. Science 348(6239):1155\u20131160","journal-title":"Science"},{"issue":"6","key":"20_CR64","doi-asserted-by":"publisher","first-page":"1066","DOI":"10.1016\/j.cell.2017.05.028","volume":"169","author":"JB Woodruff","year":"2017","unstructured":"Woodruff JB, Ferreira Gomes B, Widlund PO, Mahamid J, Honigmann A, Hyman AA (2017) The centrosome is a selective condensate that nucleates microtubules by concentrating tubulin. Cell 169(6):1066\u20131077.e10","journal-title":"Cell"},{"issue":"1650","key":"20_CR65","doi-asserted-by":"publisher","first-page":"20130459","DOI":"10.1098\/rstb.2013.0459","volume":"369","author":"JB Woodruff","year":"2014","unstructured":"Woodruff JB, Wueseke O, Hyman AA (2014) Pericentriolar material structure and dynamics. Philos Trans R Soc B: Biol Sci 369(1650):20130459","journal-title":"Philos Trans R Soc B: Biol Sci"},{"issue":"12","key":"20_CR66","doi-asserted-by":"publisher","first-page":"e323","DOI":"10.1371\/journal.pbio.0050323","volume":"5","author":"Y Xiang","year":"2007","unstructured":"Xiang Y, Takeo S, Florens L, Hughes SE, Huo L-J, Gilliland WD, Swanson SK, Teeter K, Schwartz JW, Washburn MP et al (2007) The inhibition of polo kinase by matrimony maintains G2 arrest in the meiotic cell cycle. PLoS Biol 5(12):e323","journal-title":"PLoS Biol"},{"issue":"7","key":"20_CR67","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1038\/nrm3819","volume":"15","author":"S Zitouni","year":"2014","unstructured":"Zitouni S, Nabais C, Jana SC, Guerrero A, Bettencourt-Dias M (2014) Polo-like kinases: structural variations lead to multiple functions. Nat Rev Mol Cell Biol 15(7):433\u2013452","journal-title":"Nat Rev Mol Cell Biol"}],"container-title":["EMBO Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.embopress.org\/doi\/epdf\/10.1038\/s44319-023-00020-6","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.embopress.org\/doi\/full\/10.1038\/s44319-023-00020-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.embopress.org\/doi\/epdf\/10.1038\/s44319-023-00020-6","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T01:34:37Z","timestamp":1764984877000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1038\/s44319-023-00020-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,10]]},"references-count":67,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,1,12]]}},"alternative-id":["20"],"URL":"https:\/\/doi.org\/10.1038\/s44319-023-00020-6","relation":{},"ISSN":["1469-3178"],"issn-type":[{"value":"1469-3178","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,10]]},"assertion":[{"value":"28 June 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 November 2023","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 November 2023","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 January 2024","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Disclosure and competing interests statement"}}]}}