{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:15:52Z","timestamp":1773274552089,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":1642,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Phytohemagglutinins are widely distributed in common food items. They constitute a heterogeneous group of proteins, which are often resistant to proteolysis in the gastrointestinal tract. Upon binding to the luminal membrane of intestinal cells, they can interfere with digestive, protective or secretory functions of the intestine.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Phytohemagglutinins present in red kidney beans and jackbeans have been shown to induce diarrhea and hypersecretion in human airways, but the underlying mechanisms remain obscure. We examined how agglutinins from wheat germ (WGA), soy bean (SBA), red kidney beans (Pha\u2010E, Pha\u2010L), and jackbeans (Con\u2010A) affect ion transport in mouse airways and large intestine using Ussing chamber techniques.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We found that Pha\u2010E, Pha\u2010L, and Con\u2010A but not WGA and SBA inhibit electrogenic Na<jats:sup>+<\/jats:sup> absorption dose dependently in both colon and trachea. The inhibitory effects of Con\u2010A on Na<jats:sup>+<\/jats:sup> absorption were suppressed by the sugar mannose, by inhibition of phospholipase C (PLC) and protein kinase C (PKC).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Thus, nutritional phytohemagglutinins block salt absorption in a PLC\u2010 and PKC\u2010dependent manner, probably by inhibition of the epithelial Na<jats:sup>+<\/jats:sup> channel (ENaC). This effect may be therapeutically useful in patients suffering from cystic fibrosis.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2004) <jats:bold>142<\/jats:bold>, 1219\u20131226. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0705857\">10.1038\/sj.bjp.0705857<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0705857","type":"journal-article","created":{"date-parts":[[2004,8,12]],"date-time":"2004-08-12T12:10:14Z","timestamp":1092312614000},"page":"1219-1226","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effects of dietary lectins on ion transport in epithelia"],"prefix":"10.1111","volume":"142","author":[{"given":"Karl","family":"Kunzelmann","sequence":"first","affiliation":[]},{"given":"J","family":"Sun","sequence":"additional","affiliation":[]},{"given":"R","family":"Schreiber","sequence":"additional","affiliation":[]},{"given":"Jens","family":"K\u00f6nig","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.108.1.49"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01312481"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1139\/m88-177"},{"key":"e_1_2_7_5_1","first-page":"99","article-title":"Pathogenesis of cystic fibrosis airways disease","volume":"112","author":"BOUCHER R.C.","year":"2001","journal-title":"Trans. Am. Clin. Climatol. Assoc."},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/367463a0"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.24843"},{"key":"e_1_2_7_8_1","doi-asserted-by":"crossref","first-page":"6206","DOI":"10.4049\/jimmunol.161.11.6206","article-title":"Antisense oligonucleotides targeting protein kinase C\u2010alpha, \u2010beta I, or \u2010delta but not \u2010eta inhibit lipopolysaccharide\u2010induced nitric oxide synthase expression in RAW 264.7 macrophages: involvement of a nuclear factor kappa B\u2010dependent mechanism","volume":"161","author":"CHEN C.C.","year":"1998","journal-title":"J. Immunol."},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(86)90260-X"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.290.6468.584"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.318.7190.1023"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(78)91030-9"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000199215"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)90496-6"},{"key":"e_1_2_7_15_1","first-page":"55","volume-title":"The Cystic Fibrosis Transmembrane Conductance Regulator","author":"KUNZELMANN K.","year":"2003"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.160041997"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03256658"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C100737200"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1203\/01.PDR.0000057204.51420.DC"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00232-002-1072-y"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/003655202753387347"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-011-4846-7"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.36.3.379"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306541200"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)79894-9"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108951200"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0705857","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0705857","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0705857","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T21:36:05Z","timestamp":1697232965000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0705857"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,8]]},"references-count":25,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2004,8]]}},"alternative-id":["10.1038\/sj.bjp.0705857"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0705857","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,8]]},"assertion":[{"value":"2009-01-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}