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Eighty-eight<jats:italic>E. coli<\/jats:italic>isolates belonging to phylogenetic groups B2(n = 39) and D(n = 49), including particularly widespread high risk clones or clonal complexes (HiRCC) ST131, ST69, ST393 and ST405 were studied. Spectra were analysed by unsupervised (hierarchical cluster analysis-HCA) and supervised methods (soft independent modelling of class analogy-SIMCA and partial least square discriminant analysis-PLSDA). B2-ST131 isolates were discriminated from B2 non-ST131 and D phylogroup isolates (ST69, ST393, ST405) by HCA, SIMCA and PLSDA. D-ST69, D-ST393 and D-ST405 isolates were also distinguished from each other and from other STs from phylogroup D by the three methods. We demonstrate that FTIR-ATR coupled with chemometrics is a reliable and alternative method to accurately discriminate particular<jats:italic>E. coli<\/jats:italic>clones. Its validation towards an application at a routine basis could revolutionize high-throughput bacterial typing.<\/jats:p>","DOI":"10.1038\/srep03278","type":"journal-article","created":{"date-parts":[[2013,11,20]],"date-time":"2013-11-20T10:39:40Z","timestamp":1384943980000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Diverse high-risk B2 and D Escherichia coli clones depicted by Fourier Transform Infrared Spectroscopy"],"prefix":"10.1038","volume":"3","author":[{"given":"Clara","family":"Sousa","sequence":"first","affiliation":[]},{"given":"\u00c2ngela","family":"Novais","sequence":"additional","affiliation":[]},{"given":"Ana","family":"Magalh\u00e3es","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o","family":"Lopes","sequence":"additional","affiliation":[]},{"given":"Lu\u00edsa","family":"Peixe","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2013,11,20]]},"reference":[{"key":"BFsrep03278_CR1","doi-asserted-by":"publisher","first-page":"736","DOI":"10.1111\/j.1574-6976.2011.00268.x","volume":"35","author":"N Woodford","year":"2011","unstructured":"Woodford, N., Turton, J. 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