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Not surprisingly, protein traffic disorders include a large range of diseases sharing the common mechanism of failure in the respective protein to reach the plasma membrane. However, specific therapies for these diseases are remarkably lacking. Herein, we report a robust platform for drug discovery applied to a paradigmatic genetic disorder affecting intracellular trafficking \u2013 Cystic Fibrosis. This platform includes <jats:italic>(i)<\/jats:italic> two original respiratory epithelial cellular models incorporating an inducible double-tagged traffic reporter; <jats:italic>(ii)<\/jats:italic> a plasma membrane protein traffic assay for high-throughput microscopy screening; and <jats:italic>(iii)<\/jats:italic> open-source image analysis software to quantify plasma membrane protein traffic. By allowing direct scoring of compounds rescuing the basic traffic defect, this platform enables an effective drug development pipeline, which can be promptly adapted to any traffic disorder-associated protein and leverage therapy development efforts.<\/jats:p>","DOI":"10.1038\/srep09038","type":"journal-article","created":{"date-parts":[[2015,3,12]],"date-time":"2015-03-12T10:49:40Z","timestamp":1426157380000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Protein Traffic Disorders: an Effective High-Throughput Fluorescence Microscopy Pipeline for Drug Discovery"],"prefix":"10.1038","volume":"5","author":[{"given":"Hugo M.","family":"Botelho","sequence":"first","affiliation":[]},{"given":"Inna","family":"Uliyakina","sequence":"additional","affiliation":[]},{"given":"Nikhil T.","family":"Awatade","sequence":"additional","affiliation":[]},{"given":"Maria C.","family":"Proen\u00e7a","sequence":"additional","affiliation":[]},{"given":"Christian","family":"Tischer","sequence":"additional","affiliation":[]},{"given":"Lalida","family":"Sirianant","sequence":"additional","affiliation":[]},{"given":"Karl","family":"Kunzelmann","sequence":"additional","affiliation":[]},{"given":"Rainer","family":"Pepperkok","sequence":"additional","affiliation":[]},{"given":"Margarida D.","family":"Amaral","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2015,3,12]]},"reference":[{"key":"BFsrep09038_CR1","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1002\/pmic.200900258","volume":"10","author":"L Fagerberg","year":"2010","unstructured":"Fagerberg, L., Jonasson, K., von Heijne, G., Uhlen, M. & Berglund, L. 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