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Redox mediators can be used for this purpose via different electrochemical techniques that are either complex or only provide non-continuous data. Hydrodynamic chronoamperometry using a rotating disc electrode (RDE) can alleviate these issues but was seldom used and is poorly characterized. The kinetics of<jats:italic>Faecalibacterium prausnitzii<\/jats:italic>A2-165, a beneficial gut microbe, were determined using a RDE with riboflavin as redox probe. This butyrate producer anaerobically ferments glucose and reduces riboflavin whose continuous monitoring on a RDE provided highly accurate kinetic measurements of its metabolism, even at low cell densities. The metabolic reaction rate increased linearly over a broad range of cell concentrations (9\u2009\u00d7\u200910<jats:sup>4<\/jats:sup>to 5\u2009\u00d7\u200910<jats:sup>7<\/jats:sup>cells.mL<jats:sup>\u22121<\/jats:sup>). Apparent Michaelis-Menten kinetics was observed with respect to riboflavin (K<jats:sub>M<\/jats:sub>\u2009=\u20096\u2009\u03bcM; k<jats:sub>cat<\/jats:sub>\u2009=\u20095.3\u00d710<jats:sup>5<\/jats:sup>\u2009s<jats:sup>\u22121<\/jats:sup>, at 37\u2009\u00b0C) and glucose (K<jats:sub>M<\/jats:sub>\u2009=\u20096\u2009\u03bcM; k<jats:sub>cat<\/jats:sub>\u2009=\u20092.4\u2009\u00d7\u200910<jats:sup>5<\/jats:sup>\u2009s<jats:sup>\u22121<\/jats:sup>). The short temporal resolution allows continuous monitoring of fast cellular events such as kinetics inhibition with butyrate. Furthermore, we detected for the first time riboflavin reduction by another potential probiotic,<jats:italic>Butyricicoccus pullicaecorum<\/jats:italic>. The ability of the RDE for fast, accurate, simple and continuous measurements makes it an<jats:italic>ad hoc<\/jats:italic>tool for assessing bioprocesses at high resolution.<\/jats:p>","DOI":"10.1038\/srep11484","type":"journal-article","created":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T09:20:47Z","timestamp":1435742447000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Hydrodynamic chronoamperometry for probing kinetics of anaerobic microbial metabolism \u2013 case study of Faecalibacterium prausnitzii"],"prefix":"10.1038","volume":"5","author":[{"given":"Antonin","family":"Pr\u00e9voteau","sequence":"first","affiliation":[]},{"given":"Annelies","family":"Geirnaert","sequence":"additional","affiliation":[]},{"given":"Jan B.A.","family":"Arends","sequence":"additional","affiliation":[]},{"given":"Sylvain","family":"Lanneb\u00e8re","sequence":"additional","affiliation":[]},{"given":"Tom","family":"Van de Wiele","sequence":"additional","affiliation":[]},{"given":"Korneel","family":"Rabaey","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2015,7,1]]},"reference":[{"key":"BFsrep11484_CR1","doi-asserted-by":"publisher","first-page":"1319","DOI":"10.1126\/science.240.4857.1319","volume":"240","author":"CR Myers","year":"1988","unstructured":"Myers, C. 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