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Morphological differentiation is triggered by stressful conditions and takes place in a pro-oxidant environment, which sets the basis for an involvement of the oxidative stress response in this cellular process. Characterization of the phenotypic traits of <jats:italic>Streptomyces natalensis<\/jats:italic> \u0394<jats:italic>katA1<\/jats:italic> (mono-functional catalase) and \u0394<jats:italic>catR<\/jats:italic> (Fur-like repressor of <jats:italic>katA1<\/jats:italic> expression) strains in solid medium revealed that both mutants had an impaired morphological development process. The sub-lethal oxidative stress caused by the absence of KatA1 resulted in the formation of a highly proliferative and undifferentiated vegetative mycelium, whereas de-repression of CatR regulon, from which KatA1 is the only known representative, resulted in the formation of scarce aerial mycelium. Both mutant strains had the transcription of genes associated with aerial mycelium formation and biosynthesis of the hyphae hydrophobic layer down-regulated. The first round of the programmed cell death (PCD) was inhibited in both strains which caused the prevalence of the transient primary mycelium (MI) over secondary mycelium (MII). Our data shows that the first round of PCD and morphological differentiation in <jats:italic>S. natalensis<\/jats:italic> is dependent on oxidative stress in the right amount at the right time.<\/jats:p>","DOI":"10.1038\/srep12887","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T10:46:54Z","timestamp":1439203614000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Streptomyces natalensis programmed cell death and morphological differentiation are dependent on oxidative stress"],"prefix":"10.1038","volume":"5","author":[{"given":"Tiago","family":"Beites","sequence":"first","affiliation":[]},{"given":"Paulo","family":"Oliveira","sequence":"additional","affiliation":[]},{"given":"Beatriz","family":"Rioseras","sequence":"additional","affiliation":[]},{"given":"S\u00edlvia D. 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