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Here, we build a library of hybrid collagen-polymer fibrous scaffolds with nanoscale dimensions and screen them for their ability to grow chondrocytes for cartilage repair. Poly(lactic acid) and poly (lactic-co-glycolic acid) at two different monomer ratios (85:15 and 50:50) were incrementally blended with collagen. Physical properties (wettability and stiffness) of the scaffolds were characterized and related to biological performance (proliferation, ECM production and gene expression) and structure-function relationships were developed. We found that soft scaffolds with an intermediate wettability composed of the highly biodegradable PLGA50:50 and collagen, in two ratios (40:60 and 60:40), were optimal for chondrogenic differentiation of ATDC5 cells as determined by increased ECM production and enhanced cartilage specific gene expression. Long-term cultures indicated a stable phenotype with minimal de-differentiation or hypertrophy. The combinatorial methodology applied herein is a promising approach for the design and development of scaffolds for regenerative medicine.<\/jats:p>","DOI":"10.1038\/srep14804","type":"journal-article","created":{"date-parts":[[2015,10,7]],"date-time":"2015-10-07T08:55:58Z","timestamp":1444208158000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["A combinatorial approach towards the design of nanofibrous scaffolds for chondrogenesis"],"prefix":"10.1038","volume":"5","author":[{"given":"Maqsood","family":"Ahmed","sequence":"first","affiliation":[]},{"given":"Tiago Andr\u00e9 da Silva","family":"Ramos","sequence":"additional","affiliation":[]},{"given":"Febriyani","family":"Damanik","sequence":"additional","affiliation":[]},{"given":"Bach","family":"Quang Le","sequence":"additional","affiliation":[]},{"given":"Paul","family":"Wieringa","sequence":"additional","affiliation":[]},{"given":"Martin","family":"Bennink","sequence":"additional","affiliation":[]},{"given":"Clemens","family":"van Blitterswijk","sequence":"additional","affiliation":[]},{"given":"Jan","family":"de Boer","sequence":"additional","affiliation":[]},{"given":"Lorenzo","family":"Moroni","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2015,10,7]]},"reference":[{"key":"BFsrep14804_CR1","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1038\/nrm3620","volume":"14","author":"FM Watt","year":"2013","unstructured":"Watt, F. 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