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The application of DNA barcodes has shown to be greatly useful to help delimiting species. Therefore, sequences of the cytochrome<jats:italic>c<\/jats:italic>oxidase I gene from 108 specimens of 34 morpho-species were used to investigate the molecular diversity within the gastropods from the Portuguese coast. To the above dataset, we added available COI-5P sequences of taxonomically close species, in a total of 58 morpho-species examined. There was a good match between ours and sequences from independent studies, in public repositories. We found 32 concordant (91.4%) out of the 35 Barcode Index Numbers (BINs) generated from our sequences. The application of a ranking system to the barcodes yield over 70% with top taxonomic congruence, while 14.2% of the species barcodes had insufficient data. In the majority of the cases, there was a good concordance between morphological identification and DNA barcodes. Nonetheless, the discordance between morphological and molecular data is a reminder that even the comparatively well-known European marine gastropods can benefit from being probed using the DNA barcode approach. Discordant cases should be reviewed with more integrative studies.<\/jats:p>","DOI":"10.1038\/srep20226","type":"journal-article","created":{"date-parts":[[2016,2,15]],"date-time":"2016-02-15T10:16:52Z","timestamp":1455531412000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["With a little help from DNA barcoding: investigating the diversity of Gastropoda from the Portuguese coast"],"prefix":"10.1038","volume":"6","author":[{"given":"Lu\u00edsa M. 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