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Georeferenced hyperspectral and high-resolution fluorescence imaging were coupled to microspatially mapped community composition and relative biomass of macroalgal host and epiphyte microalgal groups and their weighted contributions to productivity within host fronds of <jats:italic>Corallina officinalis<\/jats:italic> on upper and lower zones of a rocky shore were determined. Lower shore epiphytes were dominated by filamentous diatoms (Bacillariophyta), confined to the apex of the frond structure, which were low light acclimated but retained a high capacity for photoprotective down regulation and contributed up to 51% of total community productivity. Upper shore epiphytes were dominated by green algae (Chlorophyta) and single-celled diatoms (principally <jats:italic>Cocconeis<\/jats:italic> spp.), which were high light acclimated but present at far lower relative biomass and contributed negligibly to productivity. The host, <jats:italic>C. officinalis<\/jats:italic> was the main primary producer. Variation in light environment resulting from differences in shore height and shading within the host macroalga, likely play a large role in determining patterns in epiphyte community structure, biomass and productivity observed. Additionally, microspatial gradients in photophysiological parameters along the host macroalga likely resulted from age-dependent variation in pigments as well as the gradient in light environment.<\/jats:p>","DOI":"10.1038\/srep22343","type":"journal-article","created":{"date-parts":[[2016,2,29]],"date-time":"2016-02-29T11:14:46Z","timestamp":1456744486000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Microspatial variability in community structure and photophysiology of calcified macroalgal microbiomes revealed by coupling of hyperspectral and high-resolution fluorescence imaging"],"prefix":"10.1038","volume":"6","author":[{"given":"R. G.","family":"Perkins","sequence":"first","affiliation":[]},{"given":"C. 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