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Moreover, IFN-\u03b3-mediated immune memory could be evoked <jats:italic>in vitro<\/jats:italic> in parasite antigen-stimulated adipose tissue stromal vascular fraction cells collected from mice infected one year before. Innate or innate-like cells such as NK, NK T and TCR\u03b3\u03b4<jats:sup>+<\/jats:sup> cells, but also CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> TCR\u03b2<jats:sup>+<\/jats:sup> lymphocytes contributed to the IFN-\u03b3 production observed since day one of infection. This early cytokine production was largely abrogated in IL-12\/IL23 p40-deficient mice. Moreover, production of IFN-\u03b3 by stromal vascular fraction cells isolated from these mice was markedly lower than that of wild-type counterparts upon stimulation with parasite antigen. In wild-type mice the increased IFN-\u03b3 production was concomitant with up-regulated expression of genes encoding interferon-inducible GTPases and nitric oxide synthase, which are important effector molecules in controlling intracellular parasite growth. This increased gene expression was markedly impaired in the p40-deficient mice. Overall, these results show that NK cells but also diverse T cell populations mediate a prompt and widespread production of IFN-\u03b3 in the adipose tissue of <jats:italic>N. caninum<\/jats:italic> infected mice.<\/jats:p>","DOI":"10.1038\/srep23475","type":"journal-article","created":{"date-parts":[[2016,3,22]],"date-time":"2016-03-22T10:10:46Z","timestamp":1458641446000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Enrichment of IFN-\u03b3 producing cells in different murine adipose tissue depots upon infection with an apicomplexan parasite"],"prefix":"10.1038","volume":"6","author":[{"given":"Luzia","family":"Teixeira","sequence":"first","affiliation":[]},{"given":"Raquel M.","family":"Marques","sequence":"additional","affiliation":[]},{"given":"Pedro","family":"Ferreirinha","sequence":"additional","affiliation":[]},{"given":"Filipa","family":"Bezerra","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Melo","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o","family":"Moreira","sequence":"additional","affiliation":[]},{"given":"Ana","family":"Pinto","sequence":"additional","affiliation":[]},{"given":"Alexandra","family":"Correia","sequence":"additional","affiliation":[]},{"given":"Paula G.","family":"Ferreira","sequence":"additional","affiliation":[]},{"given":"Manuel","family":"Vilanova","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2016,3,22]]},"reference":[{"key":"BFsrep23475_CR1","doi-asserted-by":"publisher","first-page":"1066","DOI":"10.1128\/IAI.01455-06","volume":"75","author":"MS Desruisseaux","year":"2007","unstructured":"Desruisseaux, M. 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