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This volatile fraction comprises 428 putatively identified compounds distributed over several chemical families, being the major ones hydrocarbons, alcohols, esters, ketones and aldehydes. These metabolites may be related with different metabolic pathways, such as amino acid metabolism, biosynthesis and metabolism of fatty acids, degradation of aromatic compounds, mono and sesquiterpenoid synthesis and carotenoid cleavage. The <jats:italic>A. niger<\/jats:italic> molecular biomarkers pattern was established, comprising the 44 metabolites present in all studied conditions. This pattern was successfully used to distinguish <jats:italic>A. niger<\/jats:italic> from other fungi (<jats:italic>Candida albicans<\/jats:italic> and <jats:italic>Penicillium chrysogenum<\/jats:italic>) with 3 days of growth by using Partial Least Squares-Discriminant Analysis (PLS-DA). In addition, PLS-DA-Variable Importance in Projection was applied to highlight the metabolites playing major roles in fungi distinction; decreasing the initial dataset to only 16 metabolites. The data pre-processing time was substantially reduced and an improvement of quality-of-fit value was achieved. This study goes a step further on <jats:italic>A. niger<\/jats:italic> metabolome construction and <jats:italic>A. niger<\/jats:italic> future detection may be proposed based on this molecular biomarkers pattern.<\/jats:p>","DOI":"10.1038\/srep27441","type":"journal-article","created":{"date-parts":[[2016,6,6]],"date-time":"2016-06-06T10:04:42Z","timestamp":1465207482000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Shedding light on Aspergillus niger volatile exometabolome"],"prefix":"10.1038","volume":"6","author":[{"given":"Carina Pedrosa","family":"Costa","sequence":"first","affiliation":[]},{"given":"Diogo","family":"Gon\u00e7alves Silva","sequence":"additional","affiliation":[]},{"given":"Alisa","family":"Rudnitskaya","sequence":"additional","affiliation":[]},{"given":"Adelaide","family":"Almeida","sequence":"additional","affiliation":[]},{"given":"S\u00edlvia M.","family":"Rocha","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2016,6,6]]},"reference":[{"key":"BFsrep27441_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.2165\/00003495-200161001-00001","volume":"61","author":"G Garber","year":"2001","unstructured":"Garber, G. 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