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The etiology of glaucoma is still unknown and despite elevated intraocular pressure (IOP) being a major risk factor, the exact mechanisms responsible for RGC degeneration remain unknown. Caffeine, which is an antagonist of adenosine receptors, is the most widely consumed psychoactive drug in the world. Several evidences suggest that caffeine can attenuate the neuroinflammatory responses and afford protection upon central nervous system (CNS) injury. We took advantage of a well characterized animal model of glaucoma to investigate whether caffeine administration controls neuroinflammation and elicits neuroprotection. Caffeine or water were administered <jats:italic>ad libitum<\/jats:italic> and ocular hypertension (OHT) was induced by laser photocoagulation of the limbal veins in Sprague Dawley rats. Herein, we show that caffeine is able to partially decrease the IOP in ocular hypertensive animals. More importantly, we found that drinking caffeine prevented retinal microglia-mediated neuroinflammatory response and attenuated the loss of RGCs in animals with ocular hypertension (OHT). This study opens the possibility that caffeine or adenosine receptor antagonists might be a therapeutic option to manage RGC loss in glaucoma.<\/jats:p>","DOI":"10.1038\/srep27532","type":"journal-article","created":{"date-parts":[[2016,6,8]],"date-time":"2016-06-08T09:43:16Z","timestamp":1465378996000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["Caffeine administration prevents retinal neuroinflammation and loss of retinal ganglion cells in an animal model of glaucoma"],"prefix":"10.1038","volume":"6","author":[{"given":"Maria H.","family":"Madeira","sequence":"first","affiliation":[]},{"given":"Arturo","family":"Ortin-Martinez","sequence":"additional","affiliation":[]},{"given":"Francisco","family":"Nadal-N\u00edcolas","sequence":"additional","affiliation":[]},{"given":"Ant\u00f3nio F.","family":"Ambr\u00f3sio","sequence":"additional","affiliation":[]},{"given":"Manuel","family":"Vidal-Sanz","sequence":"additional","affiliation":[]},{"given":"Marta","family":"Agudo-Barriuso","sequence":"additional","affiliation":[]},{"given":"Ana Raquel","family":"Santiago","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2016,6,8]]},"reference":[{"key":"BFsrep27532_CR1","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1111\/j.1442-9071.2012.02773.x","volume":"40","author":"RJ Casson","year":"2012","unstructured":"Casson, R. 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